PAGE-MASTER Protein Standard (for SDS-PAGE) is a protein standard specially designed by GenScript. It consists of seven bands with molecular weight of 10 kD, 20 kD, 30 kD, 40 kD, 60 kD, 80 kD and 120 kD. All bands are highly purified, thus exhibit excellent performance in SDS-PAGE gels.
1. Non-BSA based blocking reagents (such as 5% skimmed milk) are recommended for blocking.
2. For best results, the loading volume of the marker should be optimized for different experiment conditions (from 2.5 µl to 10 µl).
3. This marker binds to the constant region of IgG, so protein A or protein G fluorephore conjugates cannot be used as the secondary antibody.
|Calculation||The apparent molecular weights of its seven protein bands are 10kD, 20kD, 30kD, 40kD, 60kD, 80kD and 120kD.|
|Storage||This product is stable at -20 °C for 12 months.|
|Volume||Load 5 µl per well for a mini-gel|
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