1. Do NOT boil this product.
2. For best results, the loading volume of the marker should be optimized for different experiment conditions (from 2.5 µl to 10 µl).
3. This marker binds to the constant region of IgG, so protein A or protein G fluorephore conjugates cannot be used as the secondary antibody.
|Calculation||The apparent molecular weights of its seven protein bands are 20 kDa, 30KDa, 40 kDa, 50KDa, 60 kDa, 80 kDa and 120 kDa.|
Store at -20°C
Avoid repeated freezing and thawing
|Volume||2.5-10 µl per well, the loading volume of the marker should be optimized for different experiment conditions|
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