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Custom stable cell line services for Bioproduction
Stable cell lines are used in a variety of research applications such as recombinant protein and antibody production, assay development, gene editing, functional studies and more. At GenScript, we offer comprehensive stable cell line development capabilities that either begin with DNA provided by our clients or that are powered by our fast, high efficiency gene synthesis service that seamlessly integrates all steps of stable cell line generation, delivering high quality stable clones of your choice. Boost your productivity and improve your results by leveraging GenScript's top quality stable cell line services.
Transient expression evaluation
Stable cell line development
We offer GLP compliant services with all 3 packages.
Our customers have the option of providing their own DNA for stable cell line generation purposes, however, GenScript recommends gene synthesis to ensure that we use high quality, codon optimized genes for the best expression levels.
GenScript allows you to choose from either Glutamine Synthetase or DHFR gene expression systems to construct your stable cell line. To start your project, DNA carrying the gene of interest must be introduced into the cell with a stable transfection protocol. Depending on your preference, we will use either plasmid-based stable transfection or lentivirus-based infection as your preferred stable transfection method.
To ensure that your protein of interest is expressed in the cells, we first characterize your protein expression by ELISA or Western Blot 48 hours after the transfection.
Transfected cells are grown in the appropriate selective media to generate stable pools. For packages using a DHFR expression system, we can also subject the stable pools to additional Methotrexate (MTX) amplification and screening, in order to increase expression levels (packages using a Glutamine Synthetase expression system do not require gene amplification). The productivity and quality of the pools is evaluated up to 10 passages for the top 10 clones.
Top 3 clones from each pool are picked and evaluated further, based on their growth rates.
Stability testing is a critical component of characterizing bioproduction cell lines. Stable cell lines in continuous culture suffer from genetic instability as the number of passages increases. Stability test will help you ensure the quality of your production cells over the intended passages.
We provide the sequence and construct report (in case of synthesized gene), top 3 stable clones and a comprehensive stable cell line generation report
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