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Protein Analytical Service

Protein Analytical Service

With world-class analytical techniques, GenScript is proud to provide a wide range of analytical testing services to ensure product quality and consistency during all stages of drug discovery and development. GenScript's experts have expertise to take both recombinant proteins and monoclonal antibodies, from research and product development through characterization and quality control tests. Services are available for both standalone analytical assays and broad testing programs, to meet your specific needs.

Key Features

Analytical services Analysis target Available method
Identification & characterization Primary structure Protein identification Western Blot
Molecular weight MALDI-TOF MS/LC-MS/SDS-PAGE/SEC-HPLC/CE-SDS
Peptide mapping RP-HPLC-UV
Sequence coverage LC-MS/MS
N-terminal/C-terminal sequence identification LC-MS/MS
Post translational modifications (PTM) Disulfide bond identification LC-MS/MS
N-glycosylation identification LC-MS/MS
N-glycan analysis HILIC-HPLC/MS
PTM analysis LC-MS/MS/HIC-HPLC
Higher order structures Higher order CD
Tm of CH2,CH3 DSC
Others pI IEF/cIEF
Charge variants cIEF/IEC-HPLC
Amino acid analysis RP-HPLC-UV/FLD
Purity & quantitation Purity SDS-PAGE/SEC-HPLC/CE-SDS
Quantitation Bradford/Lowry/BCA/UV/ELISA/AC-HPLC(protein A)
Impurity Host cell DNA(HCD) qPCR/SYBR green method
Host cell Protein(HCP) ELISA
Residual Protein A ELISA
Safety & Stability Bacterial Endotoxins Tal reagent
Microbial limit Membrane filtration
Sterile examination Membrane filtration
Abnormal toxicity  Animal test (mouse)
Mycoplasma PCR
Insoluble particles Light obscuration test
Visible particles Lamp test
Denaturation Temperature DSC

Case 1: Sequence coverage by LC-MS

  • Case sample: Antibody
  • Digestion: Trypsin, overnight
  • LC method: C18 column, 40 min Gradient
  • MS/MS mode: IDA
  • Data analysis: Protein pilot 5.0
 

Figure 1. Original mass spectrum

  • Figure 2. Deconvoluted mass spectrum

  • Figure 3. Deconvoluted mass spectrum after deglycosylation

Case 2: Glycan analysis by UHPLC-MS

  • Sample: Antibody
  • Sample type: solution in PBS
  • LC method: HILIC-HPLC column, 65 min Gradient
  • Scan Type: TOF MS
  • Reagent Kits from Waters
 


Figure 4. N-glycan types of antibodies (Trends in biotechnology, Volume 34, Issue 10, p835–846)


  • Figure 5. Total ion chromatogram of N-glycan by LC-MS method

  • Name Formula MW(labled) Charge Theoretical MW Experimental MW PPM RT(min) Intensity
    A1   1424.6 +2H 713.3 713.3 0.3 12.11 41858
    M3   1221.5 +2H 611. 611. 0.9 12.11 1630
    F(6)A1   1570.6 +2H 786.33 786.33 0.6 13.5 114406
    G0
    C67H105O37N9
    1627.7 +2H 814.84 814.84 0.8 14.02 2714
    G0F
    C73H115O41N9
    1773.7 +2H 887.87 887.87 0 15.26 2E+06
    G2-2SA
    C101H159O63N11
    2535 +2H 1268.5 1268.5 -3.2 15.29 101
    M5   1545.6 +2H 773.81 773.81 0 16.51 101
    G1a/G1b
    C73H115O42N9
    1789.7 +2H 895.86 895.86 -0.8 16.82/17.23 446
    F(6)A1G(4)1   1732.7 +2H 867.35 867.35 0.5 16.85 56477
    G0F-GN
    C81H128O46N10
    19.8 +2H 989.41 989.41 -1 17.54 4359
    G1Fa/G1Fb
    C79H125O46N9
    1935.8 +2H 968.89 968.89 -0.6 17.95/18.42 6666
    G2
    C79H125O47N9
    1951.8 +2H 9.89 9.89 -0.1 19.93 5205
    G1F-GN
    C87H138O51N10
    2138.9 +2H 1070.4 1070.4 -0.6 20.19 40
    G1F-SA
    C90H142O54N10
    2226.9 +2H 4.4 4.4 -0.5 20.27 29
    G2F
    C85H135O51N9
    2097.8 +2H 1049.9 1049.9 -0.7 20.99 1898
    G2-SA
    C90H142O55N10
    2242.9 +2H 1122.4 1122.4 -1.9 21. 1037
    F(6)M4A1G(4)1Ga(3)1
      2056.8 +2H 1029.4 1029.4 2.1 22.23 647
    G2F-GN
    C93H148O56N10
    2301.9 +2H 1152 1152 -0.5 22.94 583
    G2F-SA
    C96H152O59N10
    2389.9 +2H 1196 1196 0.1 23.03 78
    G2F-2SA
    C107H169O67N11
    2681 +2H 1341.5 1341.5 -0.7 25.26 3239


    Figure 6. 20+ N-glycan types identified in the table

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