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Reviewed (UniProtKB/Swiss-Prot)

Note: Proteins listed in ProtBank™ database are NOT off-the-shelf [catalog] proteins. Customized protein production request can be made for any protein in this database by clicking on the corresponding button under "Quick Quote". Please ensure sequence accuracy and/or make any necessary modifications prior to submitting your quote request

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Reviewed (UniProtKB/Swiss-Prot)


Name Gene Symbol Rec Full Name Protein Information Quick Quote  Price,
Turnaround
M3K5_MOUSE, Mus musculus (house mouse) Map3k5 MAPK/ERK kinase kinase 5 Functions
wild_type
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Protein Domains
mutant
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Protein Names | ProteinIDs  | Organism | Gene Names  | GeneID | Genome Annotation(Ensembl) |  Protein Sequence | 
General annotation |  References | Order
Protein Names
Recommended name:
 Mitogen-activated protein kinase kinase kinase 5;
Alternative name(s):
 Apoptosis signal-regulating kinase 1
MAPK/ERK kinase kinase 5
Protein IDs
(Accession numbers)
UniProtKB
 O35099
NCBI RefSeq
Organism
Latin Name/
(Common Name)
Mus musculus (Mouse).
Taxonomic Identifier (NCBI)
 10090
Gene Names
 Map3k5
GeneID
Genome
Annotation (Ensembl)

ENSMUST00000095806, ENSMUSP00000093485; ENSMUSG00000071369
Protein Sequence
 
SEQUENCE   1380 AA;  154512 MW;  BE68D225EBBCDF38 CRC64;
MGTEAGEGIT FSVPPFASVG FCTIPEGGSC RRGGGAATAA EGEPSLQPLL VPPPPPPPGS
FWNVESAAAP GTSCPTTAPG SSATRGRGNS GSGGGRRTTV AYVINEASQG QLVVAESEAL
QSLREACEAV GATLETLHFG KLDFGETAVL DRFYNADIAV VEMSDAFRQP SLFYHLGVRE
SFSMANNIIL YCDTNSDSLQ SLKEIICQKN TVCTGNYTFI PYMVTPHNKV YCCDSSFMKG
LTELMQPNFE LLLGPICLPL VDRFVQLLKV AQASSSQYFR ESILSDIRKA RNLYTGKELA
AELARIRQRV DNIEVLTADI VINLLLSYRD IQDYDSIVKL VETLEKLPTF DLASHHHVKF
HYAFALNRRN LPGDRAKALD IMIPMVQSEE QVASDMYCLV GRIYKDMFLD SNFTDTESRD
HGASWFKKAF ESEPTLQSGI NYAVLLLAAG HQFESSFELR KVGVKLSSLL GKKGNLEKLQ
SYWEVGFFLG ASVLANDHLR VIQASEKLFR LKTPAWYLKS IVETILIYKH FVKLTTEQPS
AKQELVDFWM DFLVEATKTD VTVVRFPVLI LEPTKIYQPS YLSINNEVEE KTISIWHVLP
DDKKGIHEWN FGASSVRGVS ISKFEERCCF LYVLHNSDDF QIYFCTELHC KRFFEMVNTI
TEEKGRGAED GDCEGDSLEY DYEYDENGDR VVLGKGTYGI VYAGRDLSNQ VRIAIKEIPE
RDSRYSQPLH EEIALHKHLK HKNIVQYLGS FSENGFIKIF MEQVPGGSLS ALLRSKWGPL
KDNEQTIGFY TKQILEGLKY LHDNQIVHRD IKGDNVLINT YSGVLKISDF GTSKRLAGIN
PCTETFTGTL QYMAPEIIDK GPRGYGKAAD IWSLGCTIIE MATGKPPFYE LGEPQAAMFK
VGMFKVHPEI PESMSAEAKA FILKCFEPDP DKRACANDLL IDEFLKVSSK KKKTQPKLSA
LSTGSNEYLR SISLPVPVLV EDTSSSSEYG SVSPDTELKA DPFSFKARAK SCGEKDGKGI
RTLFLGIPDE NFEDHSAPPS PEEKDSGFFM LRKDSERRAT LHRILTEDQD KVVRNLMESL
AQGAEEPKLK WEHITTLISS LREFVRSTDR KIIATTLSKL KLELDFDSHG ISQVQVVLFG
FQDAVNKVLR NHNIKPHWMF ALDSIIRKAV QTAITILVPE LRPHFSLASE SDTADPEDLD
VEDEHEELSS NQTVRRPQAI TEDAVATSGV STLSSTVSHD SQNAHRSLNV QLGRMKIETN
RLLEELVRKE RELQALLHQA IEEKDQEIRH LKLKSQPIDI PGFPVCHLNS PGTTTEDSEL
PGWLRENGAD EDTISRFLAE DYTLVDVLYY VTRDDLKCLR LRGGMLCTLW KAIIDFRNKC
General annotation
Function
 Component of a protein kinase signal transduction cascade. Phosphorylates and activates MAP2K4 and MAP2K6, which in turn activate the JNK and p38 MAP kinases, respectively. Overexpression induces apoptotic cell death (By similarity).
Cofactor
 Magnesium (By similarity).
Subunit structure
 Homodimer when inactive. Binds both upstream activators and downstream substrates in multimolecular complexes. Interacts with DAB2IP (By similarity). Interacts with PPM1L. Interacts with ARRB2 (By similarity).
Subcellular location

Tissue specificity
 Expressed in various adult mouse tissues including heart, brain, lung, liver and kidney.
Induction
Domain
Post-translational modification

Dephosphorylated and activated by PGAM5 (By similarity).
Involvement in disease

References

  • "Molecular cloning and characterization of the mouse apoptosis signal-regulating kinase 1.";,Tobiume K., Inage T., Takeda K., Enomoto S., Miyazono K., Ichijo H.;,Biochem. Biophys. Res. Commun. 239:905-910(1997)., PubMeb:9367868 DOI:10.1006/bbrc.1997.7580

  • Tobiume K., Inage T., Takeda K., Enomoto S., Miyazono K., Ichijo H.;,Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.

  • "The status, quality, and expansion of the NIH full-length cDNAproject: the Mammalian Gene Collection (MGC).";,Genome Res. 14:2121-2127(2004).,PubMeb:15489334 DOI:10.1101/gr.2596504

  • "Regulation of apoptosis signal-regulating kinase 1 by proteinphosphatase 2Cepsilon.";,Saito J., Toriumi S., Awano K., Ichijo H., Sasaki K., Kobayashi T.,Tamura S.;,Biochem. J. 405:591-596(2007).,PubMeb:17456047 DOI:10.1042/BJ20070231

  • "Large-scale phosphorylation analysis of mouse liver.";,Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.;,Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).,PubMeb:17242355 DOI:10.1073/pnas.0609836104

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Protein Domain Databases
InterPro

IPR011009, Kinase-like_dom.

IPR000719, Prot_kinase_cat_dom.

IPR017441, Protein_kinase_ATP_BS.

IPR017442, Se/Thr_kinase-like_dom.

IPR008271, Ser/Thr_kinase_AS.

IPR002290, Ser/Thr_kinase_dom.

IPR010993, Sterile_alpha_motif_homology.
Gene3D
PANTHER
Pfam

PF00069, Pkinase; 1.
PRINTS
SUPFAM

SSF56112, Kinase_like; 1.

SSF47769, SAM_homology; 1.
PROSITE

PS00107, PROTEIN_KINASE_ATP; 1.

PS50011, PROTEIN_KINASE_DOM; 1.

PS00108, PROTEIN_KINASE_ST; 1.
3D Structure
Database (PDB)
Entry
Method
Resolution (A)
Chain
Positions
PDBsum
Sequence
Annotation
Feature key
Position(s)
Length
Description
Polypeptide chain 1 - 1380 1380 Mitogen-activated protein kinase kinase /FTId=PRO_0000086250.
Domain 687 - 945 259 Protein kinase.
Nucleotide phosphate-binding region 693 - 701 9 ATP (By similarity).
Amino acid(s) 810 1 Proton acceptor (By similarity).
Binding 716 1 ATP (By similarity).
Posttranslational modification of a residue 843 1 Phosphothreonine (By similarity).
Posttranslational modification of a residue 845 1 Phosphothreonine (By similarity).
Posttranslational modification of a residue 965 1 Phosphoserine.
Posttranslational modification of a residue 973 1 Phosphoserine (By similarity).
Posttranslational modification of a residue 983 1 Phosphothreonine (By similarity).
Posttranslational modification of a residue 984 1 Phosphoserine (By similarity).
Posttranslational modification of a residue 985 1 Phosphoserine (By similarity).
Posttranslational modification of a residue 986 1 Phosphoserine (By similarity).
Posttranslational modification of a residue 991 1 Phosphoserine (By similarity).
Posttranslational modification of a residue 993 1 Phosphoserine (By similarity).
Posttranslational modification of a residue 996 1 Phosphothreonine (By similarity).
Posttranslational modification of a residue 1036 1 Phosphoserine (By similarity).
Posttranslational modification of a residue 1040 1 Phosphoserine (By similarity).
Different sources report differing sequences 166 1 A -> T (in Ref. 1; BAA23648).
Different sources report differing sequences 368 - 370 3 RRN -> TRT (in Ref. 1; BAA23648).
Different sources report differing sequences 1090 1 K -> N (in Ref. 1; BAA23648).
Protein-protein
Interaction
Databases
DIP
IntAct

O35099
MINT

MINT-151480
STRING

O35099
Binary Interactions
With
Entry
#Exp.
IntAct
Akt1P31750 1EBI-777493, EBI-298707
Map3k6Q9WTR2 1EBI-777493, EBI-1254790
Enzyme and Pathway Databases
Pathway
Interaction DB
Reactome
Phylogenomic Database
HOVERGEN

HBG006305
InParanoid

O35099
OMA
OrthoDB
Proteomics
Database (PRIDE)

O35099
Comparative
Toxicogenomics
Database (CTD)
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