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Phosphorylation-Specific Antibody Production Services
(Custom Antibodies for Detection of Protein Phosphorylation)

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GenScript provides customizable protein phosphorylation-specific polyclonal and monoclonal antibody services with a success rate of over 95%. Post-translational modifications of proteins, in particular, protein phosphorylation, are involved in almost all cellular events, making their detection vital for the study of numerous developmental disorders and human diseases. Our affinity-purified phospho-specific antibodies detect specific protein phosphorylation from complex cellular protein mixtures with high sensitivity. The high specificity and high sensitivity of our protein phosphorylation-specific antibodies is made possible by technological innovations including our advanced OptimumAntigen™ design tool, proprietary immunization adjuvant, optimized specificity screening and antibody purification.

Key Features of Phospho-Specific Antibody Services
(Highly Specific Antibodies for Protein Phosphorylation Detection)

• Optimized immunization: Our OptimumAntigen™ design tool and intelligent Antigen Strategy increase specificity and affinity of antibodies.
• Guaranteed results: ELISA titer of ≥1:64,000 for phospho-specific pAb and mAb
• At least 2 positive hybridoma clones for phospho-specific mAb development**
• Fast turnaround time: Only 10-11 weeks to delivery of hybridoma supernatants - 8 weeks faster than any other vendor!
• Certified facility: AAALAC International accreditation and OLAW certification, demonstrating our commitment to responsible animal care and use.

Details of Phospho-Specific Antibody Packages for Protein Phosphorylation Detection

Package	You Provide	Deliverables and Guarantees	Timeline	Quotation Request***
Polyclonal Phospho-specific Polyclonal Antibody Package (2 rabbits) (Cat. No: SC1044)	Target antigen/protein sequence	Final Deliverables 1-15 mg purified antibody 0.1 mg "pan" antibody 5 mg phosphopeptide (<15aa, 90% purity) 5 mg non-phosphopeptide (<15aa, 90% purity) 1-2 ml of pre-immune serum ELISA results (for both phosphopeptide and non-phosphopeptide), MS and HPLC reports	16 weeks
		Guarantee ELISA titer of ≥1:64,000 for purified Ab with immunogen and less than 10% cross reactivity with non-phospho peptide
Monoclonal MonoExpress™ Phospho-specific Antibody Package (5 Balb/c mice or 3 rats) (Cat. No: SC1659)	Target antigen/protein sequence	First Deliverables Up to 5 supernatants	First Delivery 10-11 weeks
		Final Deliverables Up to 5 hybridoma cell lines 2 mg purified antibody 2 mg phosphopeptide (<15aa, 90% purity) 2 mg non-phosphopeptide (<15aa, 90% purity) ELISA results (both for phosphopeptide and nonphosphopeptide), MS and HPLC reports
		Guarantees 2-5 hybridoma cell lines & at least one with ELISA titer of ≥1:64,000 for purified Ab with immunogen and less than 10% cross reactivity with non-phospho peptide
Custom poly or monoclonal phospho-specific package	Your own physical antigen	Varies with custom package design	Varies with custom design	When ready to order, physically mail with antigen

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Ordering Process for Protein Phosphorylation-Specific Antibody Services

Read protein phosphorylation-specific package protocol details.

* For collection and purification of antibodies from separated animals, a total of 2 ml of pre-immune serum (1 ml from each of the 2 animals) are provided. For antibody purification from pooled serum, 1 ml of pre-immune serum is provided.

**Guarantee applies only if peptide antigens were designed, synthesized, and conjugated by GenScript.

***Please e-mail or fax the Antigen Submission Form and quotation request (for custom packages only) to GenScript:

Antibody Services, GenScript 860 Centennial Avenue Piscataway, NJ 08854, USA

• Email: antibody@genscript.com
• Phone: 1-877-436-7274 (Toll-Free) 1-732-885-9188
• Fax: 1-732-210-0262 1-732-885-5878