|Synonyms||rHuIL-2; rHuIL2; Interleukin-2; Interleukin2|
|Description||In vitro studies performed on human cell lines demonstrate the immunoregulatory properties of Interleukin-2 (IL-2) including: 1) enhancement of lymphocyte mitogenesis and stimulation of long-term growth of human Interleukin-2 (IL-2) dependent edll lines; 2) enhancement of lymphocyte cytotoxicity; 3) induction of killer cell (lymphokine-activated (LAK) and natural (NK)) activity; 4) induction of interferon-gamma production.
Recombinant human Interleukin-2 (IL-2) is a highly purified protein with a molecular weight of approximately 15,300 Da. The chemical name is des-alanyl-1, serine-125 human interleukin-2. It is produced by recombinant DNA technology using a genetically engineered E. coli strain containing an analog of the human Human Interleukin-2 (IL-2) gene. Genetic engineering techniques were used to modify the human Interleukin-2 (IL-2) gene, and the resulting expression clone encodes a modified human Interleukin-2 (IL-2). This recombinant form differs from native Interleukin-2 (IL-2) in following ways: 1) it is not glycosylated; 2) the molecule has no N-terminal alanine; 3) the molecule has serine substituted for cysteine at amino acid position 125; 4) the aggregation state of molecule is likely to be different from that of native Interleukin-2 (IL-2).
|Specific Activity||The ED50 as determined by the dose-dependant stimulation of the proliferation of CTLL-2 was found to be < 0.1 ng/ml, corresponding to a specific activity of > 1x107 IU/mg.|
|Measured Molecular Weight||15,300 Da|
|Purity||Greater than 95% as determined by the following methods:
(a) SEC-HPLC analysis
(b) Reducing and non-reducing SDS-PAGE silver-stained gel analysis
|Formulation||The protein was lyophilized after extensive dialysis against 50mM Phosphate buffer, pH7.5, 5% Mannitol buffer.|
|Reconstitution||It is recommended to reconstitute the lyophilized recombinant human Interleukin-2 (IL-2) in sterile 18 MΩ-cm H2O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions.|
|Endotoxin Level||Less than 0.1 ng/μg (1 EU/μg) of recombinant human Interleukin-2 (IL-2) as determined by LAL test.|
|Sequence Analysis||The sequence of the first five N-terminal amino acids has been found to be Met-Pro-Thr-Ser-Ser.|
|Storage||Lyophilized recombinant human Interleukin-2 (IL-2) remains stable at room temperature for three weeks, but it is best stored desiccated below -18°C. Upon reconstitution recombinant human Interleukin-2 (IL-2) should be stored at 4°C for up to seven days. For long term storage it is recommended that a carrier protein (0.1% HSA or BSA) be added. Avoid repeated freeze-thaw cycles.|
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