|Synonyms||rHuIL-6; rHuIL6; Interleukin-6; Interleukin6|
|Description|| Interleukin-6 (IL-6) is a multifunctional protein originally discovered in the media of cells stimulated with double stranded RNA. Interleukin-6 (IL-6) induces multiple effects, as indicated by its numerous synonyms: plasmacytoma growth factor, interferon-β-2, monocyte derived human B cell growth factor, B cell stimulating factor, hepatocyte stimulating factor, Interleukin Hybridoma/Plasmacytoma-1.
Recombinant human Interleukin-6 (IL-6) that is produced in E. coli and purified by proprietary chromatographic techniques is a single, non-glycosylated polypeptide chain containing 185 amino acids and having a molecular mass of 21,000 Da.
|Specific Activity||The ED50 as determined by the dose-dependant stimulation of murine 7TD1 cells is < 0.1 ng/ml, corresponding to a Specific Activity of 5×107 IU/mg.|
PVPPGEDSKD VAAPHRQPLT SSERIDKQIR YILDGISALR
KETCNKSNMC ESSKEALAEN NLNLPKMAEK DGCFQSGFNE
ETCLVKIITG LLEFEVYLEY LQNRFESSEE QARAVQMSTK
VLIQFLQKKA KNLDAITTPD PTTNASLLTK LQAQNQWLQD
MTTHLILRSF KEFLQSSLRA LRQM
|Measured Molecular Weight||21,000 Da|
|Purity||Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC
(b) Anion-exchange FPLC
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel
|Dimers||Less than 1% as determined by silver-stained SDS-PAGE gel analysis|
|Formulation||The protein was lyophilized after extensive dialysis against 20mM HAc-NaAc, pH5.0, 150mM NaAc buffer.|
|Reconstitution||It is recommended to reconstitute the lyophilized recombinant human Interleukin-6 (IL-6) in sterile 18 MΩ-cm H2O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions.|
|Quantitation||Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.47 as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of IL-6 as a Reference Standard.
|Endotoxin Level||Less than 0.1ng/µg (1 EU/µg) determined by LAL test.|
|Sequence Analysis||The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Pro-Val-Pro-Pro.|
|Storage||Lyophilized recombinant human Interleukin-6 (IL-6) remains stable at room temperature for three weeks, but it is best stored desiccated below -18°C. Upon reconstitution recombinant human Interleukin-6 (IL-6) should be stored at 4°C for up to seven days. For long term storage it is recommended that a carrier protein (0.1% HSA or BSA) be added. Avoid repeated freeze-thaw cycles.|
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