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Catalog Products » Recombinant Proteins » Cytokine Bundles » Interleukin-6 (IL-6), Human

IL-6, Human

Synonyms rHuIL-6; rHuIL6; Interleukin-6; Interleukin6
Description Interleukin-6 (IL-6) is a multifunctional protein originally discovered in the media of cells stimulated with double stranded RNA. Interleukin-6 (IL-6) induces multiple effects, as indicated by its numerous synonyms: plasmacytoma growth factor, interferon-β-2, monocyte derived human B cell growth factor, B cell stimulating factor, hepatocyte stimulating factor, Interleukin Hybridoma/Plasmacytoma-1.

Recombinant human Interleukin-6 (IL-6) that is produced in E. coli and purified by proprietary chromatographic techniques is a single, non-glycosylated polypeptide chain containing 185 amino acids and having a molecular mass of 21,000 Da.
Species Human
Source E. coli
Biological Activity The ED50 as determined by the dose-dependant stimulation of murine 7TD1 cells is < 0.1 ng/ml, corresponding to a Specific Activity of 5×107 IU/mg.
Sequence
PVPPGEDSKD VAAPHRQPLT SSERIDKQIR YILDGISALR
KETCNKSNMC ESSKEALAEN NLNLPKMAEK DGCFQSGFNE
ETCLVKIITG LLEFEVYLEY LQNRFESSEE QARAVQMSTK
VLIQFLQKKA KNLDAITTPD PTTNASLLTK LQAQNQWLQD
MTTHLILRSF KEFLQSSLRA LRQM

Measured Molecular Weight 21,000 Da
Purity Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC
(b) Anion-exchange FPLC
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel
Dimers Less than 1% as determined by silver-stained SDS-PAGE gel analysis
Formulation The protein was lyophilized after extensive dialysis against 20mM HAc-NaAc, pH5.0, 150mM NaAc buffer.
Reconstitution It is recommended to reconstitute the lyophilized recombinant human Interleukin-6 (IL-6) in sterile 18 MΩ-cm H2O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions.
Quantitation Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.47 as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of IL-6 as a Reference Standard.
Endotoxin Level Less than 0.1ng/µg (1 EU/µg) determined by LAL test.
Sequence Analysis The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Pro-Val-Pro-Pro.
Storage Lyophilized recombinant human Interleukin-6 (IL-6) remains stable at room temperature for three weeks, but it is best stored desiccated below -18°C. Upon reconstitution recombinant human Interleukin-6 (IL-6) should be stored at 4°C for up to seven days. For long term storage it is recommended that a carrier protein (0.1% HSA or BSA) be added. Avoid repeated freeze-thaw cycles.
Note ;


Cat. No.
Product Name
Price
Z02785-10
IL-6, Rat - 10 ug
$159.00
Z02767-10
IL-6, Mouse - 10 ug
$159.00
Z02767-1
IL-6, Mouse - 1 mg
$3025.00
Z02759-10
IL-6, RhesusMacaque - 10 ug
$135.00
Z02759-1
IL-6, RhesusMacaque - 1 mg
$2300.00
Z00377-1
IL-15, Human - 1 mg
$2700.00
Z00377-50
IL-15, Human - 50 ug
$560.00
Z00377-10
IL-15, Human - 10 ug
$159.00
Z00368-1
IL-2, Human  - 1 mg
$640.00
Z00368-50
IL-2, Human  - 50 ug
$159.00
Z00368-10
IL-2, Human  - 10 ug
$65.00
 
Wernstedt I, et al. Reduced stress- and cold-induced increase in energy expenditure in IL-6 deficient mice. Am. J. Physiol. Regul. Integr. Comp. Physiol.Feb 2006

Itoh S, et al. A critical role for interleukin-6 family-mediated Stat3 activation in osteoblast differentiation and bone formation. Bone. Sep 2006; 39 (3): 505-512.

Pang Y, et al. Role of interleukin-6 in lipopolysaccharide-induced brain injury and behavioral dysfunction in neonatal rats. Neuroscience. Aug 2006; 141 (2): 745-755.

Feng R., et al. Antagonism of cannabinoid receptor 2 pathway suppresses IL-6-induced immunoglobulin IgM secretion. BMC Pharmacol Toxicol. 2014June;15:30

Chia CY., et al. Breast cancer cell invasion mediated by Galpha12 signaling involves expression of interleukins-6 and-8, and matrix metalloproteinase-2. J Mol Signal. 2014June;96

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Cat. No. Z00372
Size
Price
1 mg $2100.00