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Catalog Products » Recombinant Proteins » Cytokines » Interleukin-15 (IL-15), Human

IL-15, Human

Synonyms IL-15, MGC9721
Description Interleukin 15 (IL-15) is a cytokine that possesses a variety of biological functions. Interleukin 15 (IL-15) stimulates the proliferation of T-lymphocytes, which requires interaction of IL-15 with components of IL-2R, including IL-2R and probably IL-2R gamma, but not IL-2R.
Recombinant human Interleukin 15 (IL-15) produced in E. coli is a single, non-glycosylated polypeptide chain containing 114 amino acids and having a molecular mass of 12.5 kD.
Species Human
Source E. coli
Biological Activity The ED50 as determined by the dose-dependant stimulation of the proliferation of CTLL-2 was found to be < 0.5 ng/ml, corresponding to a specific activity of > 2x106 IU/mg.
Sequence
NWVNVISDL KKIEDLIQSM HIDATLYTES DVHPSCKVTA
MKCFLLELQV ISHESGDTDI HDTVENLIIL ANNILSSNGN
ITESGCKECE ELEEKNIKEF LQSFVHIVQM FINTS

Measured Molecular Weight 12.5 kD
Purity > 95% by SDS-PAGE and HPLC analyses.
Dimers Less than 1% as determined by silver-stained SDS-PAGE gel analysis
Formulation Recombinant human Interleukin-15 (IL-15) was lyophilized after extensive dialysis against 20mM PB, pH7.5, 150mM NaCl buffer.
Reconstitution It is recommended to reconstitute the lyophilized Recombinant Human Interleukin 15 (IL-15) in sterile 18 MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Quantitation Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.55 as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of IL-15 as a Reference Standard.
Endotoxin Level Less than 0.1 ng/µg (1 EU/µg) of recombinant human Interleukin-15 (IL-15) as determined by LAL test.
Sequence Analysis The sequence of the first five N-terminal amino acids was determined and was found to be Asn-Trp-Val-Asn-Val.
QC Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
Storage Lyophilized recombinant human Interleukin 15 (IL-15) remains stable at room temperature for three weeks, but it is best stored desiccated below -18°C. Upon reconstitution recombinant human Interleukin 15 (IL-15) should be stored at 4°C for up to seven days. For long term storage it is recommended that a carrier protein (0.1% HSA or BSA ) be added. Avoid repeated freeze-thaw cycles.
Note For in vitro use only


Cat. No.
Product Name
Price
Z00368-50
IL-2, Human  - 50 ug
$159.00
Z00372-1
IL-6, Human - 1 mg
$2100.00
 
Suzuki A, et al. Interleukin-15 increases hepatic regenerative activity. J. Hepatol. Sep 2006; 45 (3): 410-418.

Lin SJ, et al. Effect of interleukin-15 on alloreactivity in umbilical cord blood. Transpl. Immunol. Aug 2006; 16 (2): 112-116.

Waldmann TA. The biology of interleukin-2 and interleukin-15: implications for cancer therapy and vaccine design. Nat. Rev. Immunol. Aug 2006; 6 (8): 595-601.

Jennifer D,. et al Design and characterization of a protein superagonist of IL-15 fused with IL-15Rα and a high-affinity T cell receptor. Biotechnol Prog.2012 Nov-Dec;28(6):1588-97.

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Cat. No. Z00377
Size
Price
50 ug $560.00
10 ug $159.00
1 mg $2700.00
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