|Synonyms||Human Long Arg3 Insulin-like Growth Factor-I (Long-R3-IGF-I) (Receptor Grade)|
|Description||Human Long Arg3 Insulin-like Growth Factor-I (Long R3 IGF-I) is an 83 amino acid analog of human IGF-I comprising the complete human IGF-I sequence with the substitution of an Arg for the Glu at position 3 (hence R3), and a 13 amino acid extension peptide at the N-terminus. Scientists have engineered this analog with the express purpose of increasing biological activity. Human Long-R3-IGF-I is significantly more potent than human IGF-I in vitro. The enhanced potency is due to the markedly decreased binding of human Long-R3-IGF-I to IGF binding proteins which normally inhibit the biological actions of IGFs.
Recombinant human Long Arg3 Insulin-like Growth Factor-I (Long R3 IGF-I), produced in E. coli, is a single, non-glycosylated, polypeptide chain containing 83 amino acids and having a molecular mass of 9,111 Da.
|Specific Activity||The ED50 of recombinant human Long R3 IGF-I, calculated by the dose-dependant proliferation of murine BALBC 3T3 cells, is less than 1.0 ng/ml, corresponding to a specific activity of 1.0×106 IU/mg. For most in vitro applications, tjhe protein exerts its biological activity in the concentration range of 0.2-20 ng/ml.|
|Measured Molecular Weight||9,111 Da|
|Purity||The purity of GenScript Recombinant Human Long-R3-IGF-I is greater than 95% as determined by the following methods:
(a) RP-HPLC analysis
(b) Reducing and non-reducing SDS-PAGE silver-stained gel analysis
|Formulation||The protein was lyophilized after extensive dialysis against 300mM NaAc buffer.|
|Reconstitution||It is recommended that the lyophilized GenScript Long-R3-IGF-I be reconstituted in 500 mM acetic acid not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.|
|Endotoxin Level||The endotoxin level of GenScript Recombinant Human Long-R3-IGF-I is below 0.1 ng/μg (1 IEU/μg) of Long-R3-IGF-I.|
|Sequence Analysis||The sequence of the first five N-terminal amino acids has been found to be Met-Phe-Pro-Ala-Met.|
|Storage||Lyophilized recombinant recombinant human Long R3 IGF-I remains stable at room temperature for three weeks, but it is best stored desiccated below -18°C. Upon reconstitution recombinant human recombinant human Long R3 IGF-I should be stored at 4°C for up to seven days. For long term storage it is recommended that a carrier protein (0.1% HSA or BSA) be added. Avoid repeated freeze-thaw cycles.|
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