Catalog Products » Recombinant Proteins » Enzymes and Inhibitors » Enterokinase, Lyophilized, Porcine

Enterokinase, Lyophilized, Porcine

Synonyms Enterokinase; EK
Description Enterokinase (EK) is a specific protease that cleaves a protein after lysine residue at its recognition site: Asp-Asp-Asp-Asp-Lys. Enterokinase will not work if the recognition site is followed by proline. If a fusion tag is placed at the N-terminus with an enterokinase site, enterokinase will be able to remove the fusion tag and to generate the protein exactly as you need without adding any unwanted residues. GenScript's Porcine Enterokinase is a highly purified recombinant porcine enterokinase (Light Chain) expressed from P. Pastoris GS115 and is supplied in two forms for customers: a. Liquid in 50% glycerol; b. Lyophilized (glycerol free).
Species Porcine
Source P. pastoris
Specific Activity One unit is defined as the amount of enzyme needed to cleave 50 ug of fusion protein in 16 hours to 95% completion at 22°C in a buffer containing 25 mM Tris-HCl, pH 8.0.

Measured Molecular Weight Theoretical MW: 21,880 Da; The apparent MW on SDS-PAGE: about 40,000 Da.
Formulation GenScript rPorcine Enterokinase, Lyophilized has been formulated using a proprietary technology, and the enzyme can be shipped at room temperature or stand at 37°C for 7 days without losing any activity.
Storage Store at -20°C after receiving. Reconstituted EK is stable for 24 hours at 2°C - 8°C. For long term storage, store it at -20°C.

Enterokinase, Porcine
Enterokinase, Porcine

Fig.1. Each reaction contains 50 µg of purified IL-8 fusion protein and varying amount of GenScript recombinant porcine enterokinase.
The reactions were incubated at 22°C for 16 hours and analysed on a coomassie-stained SDS-PAGE (15%). Units of enzyme used per
reaction is listed below:
Lane 1. 1 U
Lane 2. 0.1 U
Lane 3. 0.01 U
Lane 4. 0.001 U
Lane 5. 0.0001 U
Lane 6. 0.00001 U
Lane 7. 0.000001 U
Lane 8. 0 U

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Cat. No. Z02199
100 IU $65.00
24 hour