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Catalog Products » Recombinant Proteins » Cytokines » Tumor Necrosis Factor-alpha (TNF-α), His, Mouse

TNF-α, His, Mouse

Synonyms Tumor Necrosis Factor-alpha (TNF-alpha), Cachectin.
Description Tumor Necrosis Factor-Alpha (TNF-alpha) plays a major role in growth regulation, differentiation, inflammation, viral replication, tumorigenesis, and autoimmune diseases. Besides inducing hemorrhagic necrosis of tumors, TNF has been found to be involved in tumorigenesis, tumor metastasis, viral replication, septic shock, fever, inflammation, and autoimmune diseases including Crohn's disease, and rheumatoid arthritis as well as graft-versus-host disease. TNF alpha-1a is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells and certain other target cells.Recombinant mouse Tumor Necrosis Factor-alpha (rmTNF-alpha) produced in E. coli is a non-glycosylated polypeptide chain of 163 amino acids. A fully biologically active molecule, rhTNF-alpha has a molecular mass of 18.3kDa analyzed by reducing SDS-PAGE and is obtained by proprietary refolding and chromatographic techniques at GenScript.
Species Mouse
Source E. coli
Biological Activity ED50<0.05ng/ml, measured in a cytotoxicity assay using L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D, corresponding to a specific activity of > 2x 107units/mg.
Sequence
MHHHHHHMLR SSSQNSSDKP VAHVVANHQV EEQLEWLSQR
ANALLANGMD LKDNQLVVPA DGLYLVYSQV LFKGQGCPDY
VLLTHTVSRF AISYQEKVNL LSAVKSPCPK DTPEGAELKP
WYEPIYLGGV FQLEKGDQLS AEVNLPKYLD FAESGQVYFG
VIAL

Measured Molecular Weight 18.3kDa, observed by reducing SDS-PAGE.
Purity > 95% as analyzed by reducing SDS-PAGE.
Formulation Lyophilized after extensive dialysis against PBS.
Reconstitution Reconstituted in ddH2O at 100 μg/ml.
Endotoxin Level <1 EU/μg, determined by LAL method.
Storage Lyophilized recombinant mouse Tumor Necrosis Factor-alpha (rmTNF-slpha) remains stable up to 12 months at lower than -70°C from date of receipt. Upon reconstitution, rmTNF-alpha should be stable up to 4 week at 4°C or up to 6 months at -20°C.
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Cat. No. Z02684
Size
Price
100 ug $259.00
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