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Granulocyte Macrophage Colony Stimulating Factor (GM-CSF), Human (P. pastoris-expressed)
GM-CSF, Human (P. pastoris-expressed)
| Synonyms | Granulocyte Macrophage Colony Stimulating Factor, CSF-2, MGI-1GM, GM-CSF, Pluripoietin-alpha, Molgramostin, Sargramostim, MGC131935, MGC138897 |
| Description | Human Granulocyte Macrophage Colony Stimulating Factor (hGM-CSF), a hematopoietic growth factor, is mainly involved in granulopoiesis and monocytopoiesis. It is produced by T-cells and macrophages in response to antigens, and by endothelial cells and fibroblasts following induction of variouscytokines [1]. A monomeric protein of 127 amino acidswith six glycosylation sites and two intra disulfide bonds[2,3], glycosylated and non-glycosylated hGM-CSFs show similar biological activities[4]. Other than its connection to the growth and development of granulocytes and macrophages, it is also indispensable for the proliferation of erythroid and megakaryocytic cells[5]. Recombinant Human Granulocyte Macrophage Colony Stimulating Factor (rhGM-CSF) produced in P. pastoris a glycosylated polypeptide. A fully biologically active molecule, rhGM-CSF has a molecular mass of 26-32kDa analyzed by SDS-PAGE and is obtained by proprietary refolding and chromatographic techniques at GenScript. |
| Species | Human |
| Source | P. pastoris |
| Biological Activity | ED50 < 0.1ng/ml, measured by proliferation assay of TF-1 cells, corresponding to a specific activity of > 1.0x 107 units/mg. |
| Sequence | MAPARSPSPS TQPWEHVNAI QEARRLLNLS RDTAAEMNET VEVISEMFDL QEPTCLQTRL ELYKQGLRGS LTKLKGPLTM MASHYKQHCP PTPETSCATQ IITFESFKEN LKDFLLVIPF DCWEPVQE |
| Measured Molecular Weight | 26-32 kDa, observed by SDS-PAGE. |
| Purity | > 95 % as analyzed by SDS-PAGE. |
| Formulation | Lyophilized after extensive dialysis against 10 mM PB, pH7.0. |
| Reconstitution | Reconstituted in ddH2O at 100μg/ml. |
| Endotoxin Level | <1.0 EU/μg, determined by LAL method. |
| Storage | Lyophilized recombinant human Granulocyte Macrophage Colony Stimulating Factor (rhGM-CSF) remains stable up to 12 months at lower than -70°C from date of receipt. Upon reconstitution, rhGM-CSF should be stable up to 4 weeks at 4°C or up to 6 months at -20°C. |
ED₅₀< 0.2ng/ml, measured by proliferation assay of TF-1 cells, corresponding to a specific activity of > 5x 10⁶ units/mg.
5. Hercus, T. R. et al. (2012) Growth Factors. 30(2):63-75.
3. Hansen, G.et al. (2008) Cell. 134:496-507.
1. Hamilton, J. A. et al. (2005) Growth Factors. 22(4): 225–31.
You can visit "Citations Database" for more citations.
3. Hansen, G.et al. (2008) Cell. 134:496-507.
1. Hamilton, J. A. et al. (2005) Growth Factors. 22(4): 225–31.
You can visit "Citations Database" for more citations.
For more documents, please visit "Technical Support".
| Cat. No. Z02694 | ||
|---|---|---|
| Size | Price | |
| 1 mg | $2100.00 | |
