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Catalog Products » Recombinant Proteins » Catalog Proteins - View by Cat. No. » Thymic Stromal Lymphopoietin (TSLP), Human

TSLP, Human

Synonyms Thymic Stromal Lymphopoietin ( TSLP), Human;
Description TSLP is a hemopoietic protein that is expressed in the heart, liver and prostate. TSLP overlaps biological activities with IL-7 and binds with the heterodimeric receptor complex consisting of the IL-7R alpha chain (IL-7Rª) and the TSLP-specific chain (TSLPR). Like IL-7, TSLP induces phosphorylation of STAT3 and STAT5, but uses kinases other than the JAKs for activation. TSLP prohibited apoptosis and stimulated growth of the human acute myeloid leukemia (AML)-derived cell line MUTZ3. It induces the release of T cell-attracting chemokines TARC and MDC from monocytes and activates CD11c(+) dendritic cells (DCs). TSLP activated DCs primed naive T cells to produce the proallergic cytokines (IL-4, IL-5, IL-13, TNFª) while down-regulating IL-10 and IFN-γ suggesting a role in initiating allergic inflammation.
Species Human
Source E. coli
Biological Activity Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human IL-7Rα and human TSLP R co-transfected murine BaF3 pro-B cells is less than 0.3 ng/ml, corresponding to a specific activity of > 3.3 × 106 IU/mg.
Sequence
MYDFTNCDFE KIKAAYLSTI SKDLITYMSG TKSTEFNNTV
SCSNRPHCLT EIQSLTFNPT AGCASLAKEM FAMKTKAALA IWCPGYSETQ
INATQAMKKR RKRKVTTNKC LEQVSQLQGL WRRFNRPLLK QQ

Measured Molecular Weight Approximately 15.1 kDa, a single non-glycosylated polypeptide chain containing 132 amino acids.
Purity > 98 % by SDS-PAGE and HPLC analyses.
Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Endotoxin Level Less than 1 EU/μg of rHuTSLP as determined by LAL method.
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Usage This material is for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
Note ;




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Cat. No. Z02721
Size
Price
10 ug $159.00
1 mg $3600.00