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Catalog Products » Recombinant Proteins » Catalog Proteins - View by Cat. No. » Cytokines and Growth Factors » Interleukin-3 (IL-3), Mouse

IL-3, Mouse

Synonyms Interleukin-3 ( IL-3 ), Mouse;
Description IL-3 is a hematopoietic growth factor that promotes the survival, differentiation and proliferation of committed progenitor cells of the megakaryocyte, granulocyte-macrophage, erythroid, eosinophil, basophil and mast cell lineages. Produced by T cells, mast cells and eosinophils, IL-3 enhances thrombopoieses, phagocytosis, and antibody-mediated cellular cytotoxicity. Its ability to activate monocytes suggests that IL-3 may have additional immunoregulatory roles. Many of the IL-3 activities depend upon co-stimulation with other cytokines. IL-3 is species-specific, variably glycosylated cytokine.
Species Mouse
Source E. coli
Specific Activity Fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of the proliferation of murine M-NFS-60 cells is less than 0.05 ng/ml, corresponding to a specific activity of > 2 × 107 IU/mg.
Sequence
DTHRLTRTLN CSSIVKEIIG KLPEPELKTD DEGPSLRNKS
FRRVNLSKFV ESQGEVDPED RYVIKSNLQK LNCCLPTSAN DSALPGVFIR
DLDDFRKKLR FYMVHLNDLE TVLTSRPPQP ASGSVSPNRG TVEC

Measured Molecular Weight Approximately 14.8 kDa globular protein containing 134 amino acid residues.
Purity > 98 % by SDS-PAGE and HPLC analyses.
Formulation Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.4.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Endotoxin Level Less than 1 EU/μg of rMuIL-3 as determined by LAL method.
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Usage This material is for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
Note ;




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Cat. No. Z02765
Size
Price
10 ug $175.00
1 mg $3275.00
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