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Catalog Products » Recombinant Proteins » Growth Factors » Fibroblast Growth Factor-basic (FGF-basic), Rat

FGF-basic, Rat

Synonyms Fibroblast Growth Factor-basic ( FGF-basic ), Rat;
Description FGF-basic is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-basic is a non-glycosylated heparin binding growth factor that is expressed in the brain, pituitary, kidney, retina, bone, testis, adrenal gland liver, monocytes, epithelial cells and endothelial cells. FGF-basic signals through FGFR 1b, 1c, 2c, 3c and 4.
Species Rat
Source E. coli
Biological Activity Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine balb/c 3T3 cells is less than 0.2 ng/ml, corresponding to a specific activity of > 5.0 × 106 IU/mg.
Sequence
MPALPEDGGG AFPPGHFKDP KRLYCKNGGF FLRIHPDGRV
DGVREKSDPH VKLQLQAEER GVVSIKGVCA NRYLAMKEDG RLLASKCVTE
ECFFFERLES NNYNTYRSRK YSSWYVALKR TGQYKLGSKT GPGQKAILFL
PMSAKS

Measured Molecular Weight Approximately 16.4 kDa, a single non-glycosylated polypeptide chain containing 146 amino acids.
Purity > 98 % by SDS-PAGE and HPLC analyses.
Formulation Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.4.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Endotoxin Level Less than 1 EU/μg of rRtbFGF as determined by LAL method.
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Usage This material is for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
Note ;


Cat. No.
Product Name
Price
Z03310-10
FGF-basic (145aa), Human - 10 ug
$40.00
Z03166-10
FGF-basic (146aa), Human - 10 ug
$39.00
Z03116-10
FGF-basic (154aa), Human - 10 ug
$40.00
Z03230-10
FGF-basic, Bovine - 10 ug
$40.00
Z03016-1
FGF-basic, Mouse - 1 mg
$625.00
Z03193-10
FGF-basic, Rat - 10 ug
$40.00
 



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Cat. No. Z02786
Size
Price
50 ug $159.00