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Catalog Products » Recombinant Proteins » Catalog Proteins - View by Cat. No. » Interferon-tau (IFN-tau), Ovine

IFN-tau, Ovine

Synonyms Interferon-tau ( IFN-tau ), Ovine;
Description IFN-tau is a new class of type I IFN that is secreted by the trophoblast and is the signal for maternal recognition of pregnancy in sheep. IFN- tau has potent immunosuppressive and antiviral activities similar to other type I IFN but is less cytotoxic than IFN-ª/ß. The current investigation concerns the effect of recombinant ovine IFN- tau (rOvIFN- tau) on the modulation of MHC class I and II expression on cloned mouse cerebrovascular endothelial (CVE) cells. IFN- tau induced tyrosine phosphorylation of Stat1 and upregulated the expression of MHC class I on CVE. One proposed action by which type I IFN reduce the relapse rate in MS is via interference with IFN-γ-induced MHC class II expression. IFN- tau was shown to downregulate IFN-γ-induced MHC class II expression on CVE and, hence, may be of potential therapeutic value in downregulating inflammation in the central nervous system (CNS). IFN- tau did not upregulate the expression of MHC class II on CVE. IFN- tau also inhibited the replication of Theiler's virus in CVE.
Species Ovine
Source P. pastoris
Biological Activity Fully biologically active when compared to IFN-alpha. The specific activity determined by a viral resistance assay is no less than 1.0 × 107 IU/mg.
Sequence
CYLSRKLMLD ARENLKLLDR MNRLSPHSCL QDRKDFGLPQ
EMVEGDQLQK DQAFPVLYEM LQQSFNLFYT EHSSAAWDTT
LLEQLCTGLQ QQLDHLDTCR GQVMGEEDSE LGNMDPIVTV
KKYFQGIYDY LQEKGYSDCA WEIVRVEMMR ALTVSTTLQK
RLTKMGGDLN SP

Measured Molecular Weight Approximately 19.9 kDa, a single non-glycosylated polypeptide chain containing 172 amino acids.
Purity > 95 % by SDS-PAGE and HPLC analyses.
Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.
Endotoxin Level Less than 1 EU/μg of rOvIFN-τ as determined by LAL method.
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Usage This material is for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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Cat. No. Z02790
Size
Price
10 ug $159.00
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