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Catalog Products » Recombinant Proteins » Catalog Proteins - View by Cat. No. » MIC-A, Human

MIC-A, Human

Synonyms MIC-A, Human;
Description MIC-A (MHC class I chain-related gene A) is a transmembrane glycoprotein that functions as a ligand for human NKG2D. A closely related protein, MICB, shares 85% amino acid identity with MICA. These proteins are distantly related to the MHC class I proteins. They possess three extracellular Ig-like domains, but they have no capacity to bind peptide or interact with ß2-microglobulin. The genes encoding these proteins are found within the Major Histocompatibility Complex on human chromosome 6. The MICA locus is highly polymorphic with more than 50 recognized human alleles. MICA is absent from most cells but is frequently expressed in epithelial tumors and can be induced by bacterial and viral infections. MICA is a ligand for human NKG2D, an activating receptor expressed on NK cells, NKT cells, γδ T cells, and CD8+ ªß T cells. Recognition of MICA by NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. MICA recognition is involved in tumor surveillance, viral infections, and autoimmune diseases.
Species Human
Source E. coli
Biological Activity Fully biologically active when compared to standard. The specific activity is determined by binding MICA antibody in ELISA.
Sequence
EPHSLRYNLT VLSWDGSVQS GFLAEVHLDG QPFLRYDRQK
CRAKPQGQWA EDVLGNKTWD RETRDLTGNG KDLRMTLAHI
KDQKEGLHSL QEIRVCEIHE DNSTRSSQHF YYDGELFLSQ
NLETEEWTVP QSSRAQTLAM NVRNFLKEDA MKTKTHYHAM
HADCLQELRR YLESGVVLRR TVPPMVNVTR SEASEGNITV
TCRASSFYPR NIILTWRQDG VSLSHDTQQW GD

Measured Molecular Weight Approximately 32.8 kDa, a single non-glycosylated polypeptide chain containing 285 amino acids.
Purity > 95 % by SDS-PAGE and HPLC analyses.
Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Endotoxin Level Less than 1 EU/μg of rHuMIC-A, His as determined by LAL method.
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Usage This material is for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
Note ;


Cat. No.
Product Name
Price
Z02801-1
MIC-B, Human - 1 mg
$1250.00
 


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Cat. No. Z02800
Size
Price
50 ug $135.00
1 mg $1250.00