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Catalog Products » Recombinant Proteins » Other Catalog Proteins » High-Mobility Group Box 1 (HMGB1), His , Human

HMGB1, His, Human

Synonyms High-Mobility Group Box 1 (His6-tagged) ( HMGB1), Human;
Description Human High-mobility group box 1 protein (HMGB1), previously known as HMG-1 or amphoterin, is a member of the high mobility group box family of non-histone chromosomal proteins. Human HMGB1 is expressed as a 30 kDa, 215 amino acid (aa) single chain polypeptide containing three domains: two N-terminal globular, 70 aa positively charged DNA-binding domains (HMG boxes A and B), and a negatively charged 30 aa C-terminal region that contains only Asp and Glu.4, 5 Residues 27 - 43 and 178 - 184 contain a NLS. Posttranslational modifications of the molecule have been reported, with acetylation occurring on as many as 17 lysine residues. HMGB1 is expressed at high levels in almost all cells. It was originally discovered as a nuclear protein that could bend DNA. Such bending stabilizes nucleosome formation and regulates the expression of select genes upon recruitment by DNA binding proteins.
Species Human
Source E. coli
Sequence
MGKGDPKKPR GKMSSYAFFV QTCREEHKKK HPDASVNFSE
FSKKCSERWK TMSAKEKGKF EDMAKADKAR YEREMKTYIP PKGETKKKFK
DPNAPKRPPS AFFLFCSEYR PKIKGEHPGL SIGDVAKKLG EMWNNTAADD
KQPYEKKAAK LKEKYEKDIA AYRAKGKPDA AKKGVVKAEK SKKKKEEEED
EEDEEDEEEE EDEEDEDEEE DDDDELEHHH HHH

Measured Molecular Weight Approximately 26.0 kDa, a single non-glycosylated polypeptide chain containing 223 amino acids with 6 × His at C-terminus.
Purity > 95 % by SDS-PAGE and HPLC analyses.
Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Endotoxin Level Less than 1 EU/μg of rHuHMGB1, His as determined by LAL method.
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Usage This material is for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
Note ;


Contreras V, .et al Cap-independent translation promotes C. elegans germ cell apoptosis through Apaf-1/CED-4 in a caspase-dependent mechanism. PLoS One. 2011 6(9):e24444.

Dikeakos JD, .et al An interdomain binding site on HIV-1 Nef interacts with PACS-1 and PACS-2 on endosomes to down-regulate MHC-I. Mol Biol Cell. 2012 Jun;23(11):2184-97.

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Cat. No. Z02803
Size
Price
50 ug $135.00
1 mg $1250.00