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Catalog Products » Recombinant Proteins » Chemokines » MIP-3/CCL23, Human

MIP-3/CCL23, Human



Synonyms MIP-3/CCL23, Human;
Description MIP-3/CCL23 is a CC chemokine that signals through the CCR1 receptor. MIP-3 chemoattracts monocytes, resting T-lymphocytes and neutrophils, but does not chemoattract activated lymphocytes. Additionally, MIP-3 has been shown to inhibit colony formation of bone marrow myeloid immature progenitors. Alternative splicing of the MPIF1 gene results in two mRNAs that encode a short (CKß8) and a long (CKß81) isoform of the chemokine. CKß8 cDNA encodes a 120 amino acid (aa) residue precursor protein with a putative 21 aa residue signal peptide that is cleaved to generate a 99 aa residue mature CKß8 (aa 22 120). Additional N terminal processing of the 99 aa residue variant can generate a 75 aa residue CKß8 (aa 46 120) that is significantly more active than the 99 aa residue variant.
Species Human
Source E. coli
Specific Activity Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration of 10-50 ng/ml.
Sequence
RVTKDAETEF MMSKLPLENP VLLDRFHATS ADCCISYTPR
SIPCSLLESY FETNSECSKP GVIFLTKKGR RFCANPSDKQ VQVCMRMLKL
DTRIKTRKN

Measured Molecular Weight Approximately 11.4 kDa, a single, non-glycosylated polypeptide chain containing 99 amino acids.
Purity > 97 % by SDS-PAGE and HPLC analyses.
Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Endotoxin Level Less than 1 EU/μg of rHuMIP-3/CCL23 as determined by LAL method.
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Usage This material is for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.




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Cat. No. Z02845
Size
Price
20 ug $159.00
1 mg $2100.00
24 hour
 
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