×
Catalog Products » Recombinant Proteins » Catalog Proteins - View by Cat. No. » Cytokines and Growth Factors » Beta Defensins-2 (BD-2), Mouse

BD-2, Mouse

Synonyms BD 2 Murine ; BD-2 Murine
Description Defensins (alpha and beta) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. The α-defensins are distinguished from the β-defensins by the pairing of their three disulfide bonds. To date, four β-defensins have been identified; BD-1, BD-2, BD-3 and BD-4. β-defensins are expressed on some leukocytes and at epithelial surfaces. In addition to their direct antimicrobial activities, they are chemoattractant towards immature dendritic cells and memory T cells. The β-defensin proteins are expressed as the C-terminal portion of precursors and are released by proteolytic cleavage of a signal sequence and, in the case of BD-1 (36 a.a.), a propeptide region. β-defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds.
Species Mouse
Source E. coli
Specific Activity Fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using immature human dendritic cells is in a concentration of 10-100 ng/ml.
Sequence
AVGSLKSIGY EAELDHCHTN GGYCVRAICP PSARRPGSCF
PEKNPCCKYM K

Measured Molecular Weight Approximately 5.5 kDa, a single non-glycosylated polypeptide chain containing 51 amino acids.
Purity > 98 % by SDS-PAGE and HPLC analyses.
Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Endotoxin Level Less than 1 EU/μg of rMuBD-2 as determined by LAL method.
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
Note ;




Notice there is no results found, you can visit "Citations Database" for other results.







For more documents, please visit "Technical Support".

Cat. No. Z02894
Size
Price
20 ug $159.00
1 mg $2100.00
*
*
*
*