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Catalog Products » Recombinant Proteins » Catalog Proteins - View by Cat. No. » Interleukin-1 alpha (IL-1α), Mouse

IL-1α, Mouse

Synonyms Interleukin-1 Alpha, Mouse;
Description Interleukin-1 alpha (IL-1α) is a non-secreted proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1αand IL-1β binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1βis a secreted cytokine, IL-1αis predominantly a cell-associated cytokine.
Species Mouse
Source E. coli
Biological Activity Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 2 pg/ml, corresponding to a specific activity of > 5.0 × 108 IU/mg.
Sequence
SAPYTYQSDL RYKLMKLVRQ KFVMNDSLNQ TIYQDVDKHY
LSTTWLNDLQ QEVKFDMYAY SSGGDDSKYP VTLKISDSQL FVSAQGEDQP
VLLKELPETP KLITGSETDL IFFWKSINSK NYFTSAAYPE LFIATKEQSR
VHLARGLPSM TDFQIS

Measured Molecular Weight Approximately 17.9 kDa, a single non-glycosylated polypeptide chain containing 156 amino acids.
Purity > 97 % by SDS-PAGE and HPLC analyses.
Formulation Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.4.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Endotoxin Level Less than 1 EU/μg of rMuIL-1α as determined by LAL method.
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
Note ;




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Cat. No. Z02912
Size
Price
10 ug $159.00
1 mg $3600.00