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Catalog Products » Recombinant Proteins » Growth Factors » Macrophage Colony Stimulating Factor (M-CSF), Human

M-CSF, Human

M-CSF, Human

Biological Activity
M-CSF, Human (Cat. No. Z02914) stimulates cell proliferation of M-NFS-60 cells. The ED50 for this effect is typically 1-3ng/mL.

M-CSF, Human

2 μg of M-CSF, Human (Cat. No. Z02914) was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie Blue staining.

Synonyms Macrophage Colony Stimulating Factor, CSF-1, Lanimostim, MCSF, MGC31930, M-CSF.
Description Macrophage Colony-Stimulating Factor 1 (M-CSF), involved especially in monocytopoiesis,[1]is a hematopoietic growth factor. In mammals, it exits three isoforms, which invariably share an N-terminal 32-aa signal peptide, a 149-residue growth factor domain, a 21-residue transmembrane region and a 37-aa cytoplasmictail[2]. The biological activity of human M-CSF is maintained within the 149-aa growth factor domain[3], and it is only active in the disulfide-linked dimeric form[4], which is bonded at Cys63.

Recombinant human Macrophage Colony-Stimulating Factor 1 (rhM-CSF) produced in E. coli is a disulfide-linked homodimer containing two non-glycosylated polypeptide chains of 159 amino acids each. A fully biologically active molecule, rhM-CSF has a molecular mass of 28 kDaanalyzed by non-reducing SDS-PAGE and is obtained by proprietary refolding and chromatographic techniques at GenScript.
Species Human
Source E. coli
Biological Activity ED50 of 1 - 3 ng/ml, measured by cell proliferation assay of M-NFS-60, corresponding to a specific activity of 3.3 x 105-1 x 106 units/mg.
Sequence
MEEVSEYCSH MIGSGHLQSL QRLIDSQMET SCQITFEFVD
QEQLKDPVCY LKKAFLLVQD IMEDTMRFRD NTPNAIAIVQ
LQELSLRLKS CFTKDYEEHD KACVRTFYET PLQLLEKVKN
VFNETKNLLD KDWNIFSKNC NNSFAECSSQ GHERQSEGS

Measured Molecular Weight 28 kDa, observed by non-reducing SDS-PAGE.
Purity > 95% as analyzed by non-reducing SDS-PAGE.
Formulation Lyophilized after extensive dialysis against 50 mM Tris-HCl, pH 8.0.
Reconstitution Reconstituted in ddH2O or PBS or Tris-HCl, pH 8.0 at 100 μg/ml.
Endotoxin Level <1 EU/μg, determined by LAL method.
Storage Lyophilized recombinant human Macrophage Colony-Stimulating Factor 1 (rhM-CSF) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, rhM-CSF should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.

  • M-CSF, Human
  • M-CSF, Human

    Biological Activity
    M-CSF, Human (Cat. No. Z02914) stimulates cell proliferation of M-NFS-60 cells. The ED50 for this effect is typically 1-3ng/mL.

  • M-CSF, Human
  • M-CSF, Human

    2 μg of M-CSF, Human (Cat. No. Z02914) was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie Blue staining.


Bertin J,. et al Leukotrienes inhibit early stages of HIV-1 infection in monocyte-derived microglia-like cells. J Neuroinflammation. 2012 Mar 16;9:55.

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Cat. No. Z02914
Size
Price
10 ug $85.00
100 ug $450.00
1 mg $2100.00