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Catalog Products » Recombinant Proteins » Cytokines » Interferon-gamma (IFN-γ), Human

IFN-γ, Human

IFN-γ, Human

Biological Activity
IFN-gamma, Human (Cat. No. Z02915) induced cytotoxicity of HT-29 (HTB-38) cells. The ED50 for this effect is less than 0.05ng/mL.

IFN-γ, Human

2 μg of IFN-γ, Human (Cat. No. Z02915) was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie Blue staining.

Synonyms Immune Interferon, type II interferon, T cell interferon, MAF, IFNG, IFG, IFI,IFN-gamma.
Description Human Interferon gamma (hIFN-γ) is amacrophage‐activating factor and the lone member of Interferon type II.The active form of IFN-γ is an antiparallel dimer that interacts with the receptor IFN-γR1 and sets off IFN-γ/JAK/STAT pathway. IFN-γ signaling does diverse biological functions primarily related to host defense and immune regulation, including antiviral and antibacterial defense, apoptosis, inflammation, and innate and acquired immunity. While IFN-γ–induced inflammatory cascade summons a variety of immune‐related cell types, such as macrophages, natural killer (NK) cells and cytotoxic T lymphocytes (CTLs), IFN-γ is also implicated in resistance to NK cell and CTL responses and in immune escape in a variety of cancers.

Recombinant human Interferon gamma (rhIFN-γ) produced in E. coli is a non‐glycosylated polypeptide chain of 144 amino acids. A fully biologically active molecule, rhIFN-γ has a molecular mass of 17 kDa analyzed by reducing SDS‐PAGE and is obtained by proprietary refolding and chromatographic techniques at GenScript.
Species Human
Source E. coli
Specific Activity ED50<0.05ng/ml, measured by cytotoxicity assay using HT‐29 cells.
Sequence
MQDPYVKEAE NLKKYFNAGH SDVADNGTLF LGILKNWKEE
SDRKIMQSQI VSFYFKLFKN FKDDQSIQKS VETIKEDMNV
KFFNSNKKKR DDFEKLTNYS VTDLNVQRKA IHELIQVMAE
LSPAAKTGKR KRSQMLFRGR RASQ

Measured Molecular Weight 17kDa, observed by reducing SDS‐PAGE.
Purity > 95% as analyzed by reducing SDS‐PAGE.
Formulation Lyophilized after extensive dialysis against PBS.
Reconstitution Reconstituted in ddH2O or PBS at 100 μg/ml.
Endotoxin Level <1 EU/μg, determined by LAL
Sequence Analysis Gln24‐Gln166 (accession number:P01579). Expression construct with an N‐terminal Met.
Storage Lyophilized recombinant human Interferon gamma (rhIFN‐γ) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhIFN-γ should be stable up to 1 week at 4°C or up to 2 months at -20°C.

  • IFN-γ, Human
  • IFN-γ, Human

    Biological Activity
    IFN-gamma, Human (Cat. No. Z02915) induced cytotoxicity of HT-29 (HTB-38) cells. The ED50 for this effect is less than 0.05ng/mL.

  • IFN-γ, Human
  • IFN-γ, Human

    2 μg of IFN-γ, Human (Cat. No. Z02915) was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie Blue staining.


Trinchieri, G.(2010) J Exp Med. 207:2053–63.

Hu, X. et al. (2008) Immunol Rev. 226: 41–56.

Wischhusen, J. et al.(2007) Semin Cancer Biol. 17:459–68.

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Cat. No. Z02915
Size
Price
50 ug $60.00
100 ug $85.00
10 ug $25.00
1 mg $425.00
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