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Catalog Products » Recombinant Proteins » Cytokines » Interferon-gamma (IFN-γ), Mouse

IFN-γ, Mouse

Synonyms Type II interferon, T cell interferon, MAF, IFNG, IFG, IFI
Description Sharing 41% sequence identity with human Interferon gamma (hIFN--γ), mouse IFN gamma (mIFN--γ)is a macrophage-activating factor.The active form of IFN--γ is an antiparallel dimer that sets off IFN--γ/JAK/STAT pathway. IFN--γ signaling does diverse biological functions primarily related to host defense and immune regulation, including antiviral and antibacterial defense, apoptosis, inflammation, and innate and acquired immunity.While IFN--γ–induced inflammatory cascade summons a variety of immune-related cell types, such as macrophages, natural killer (NK) cells and cytotoxic T lymphocytes (CTLs), IFN--γ is also implicated in resistance to NK cell and CTL responses and in immune escape in avariety of cancers.

Recombinant mouse IFN gamma (rmIFN-γ) produced in E. coli is a non-glycosylated polypeptide chain of 134 amino acids. A fully biologically active molecule, rmIFN--γ has a molecular mass of 15 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary refolding and chromatographic techniques at GenScript.
Species Mouse
Source E. coli
Specific Activity ED50<0.15ng/ml, measured by cytotoxicity assay using WEHI-279 cells.
Sequence
MHGTVIESLE SLNNYFNSSG IDVEEKSLFL DIWRNWQKDG
DMKILQSQII SFYLRLFEVL KDNQAISNNI SVIESHLITT
FFSNSKAKKD AFMSIAKFEV NNPQVQRQAF NELIRVVHQL
LPESSLRKRK RSRC

Measured Molecular Weight 15 kDa, observed by reducing SDS-PAGE.
Purity > 95% as analyzed by reducing SDS-PAGE.
Formulation Lyophilized after extensive dialysis against PBS.
Reconstitution Reconstituted in ddH2O or PBS at 100 μg/ml.
Endotoxin Level <1 EU/μg, determined by LAL method.
Storage Lyophilized recombinant mouse IFN gamma (rmIFN-γ) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rmIFN-γ should be stable up to 1week at 4°C or up to 2 months at -20°C.

  • IFN-γ, Mouse
  • IFN-γ, Mouse

    ED₅₀<0.15ng/ml, measured by cytotoxicity assay using WEHI-279 cells.


Trinchieri, G. (2010) J Exp Med. 207:2053–63.

Hu, X. et al. (2008) Immunol Rev. 226: 41–56.

Wischhusen, J. et al. (2007) Semin Cancer Biol. 17:459–68.

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Cat. No. Z02916
Size
Price
500 ug $285.00
20 ug $40.00
100 ug $85.00
1 mg $425.00
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