|Synonyms||Type II interferon, T cell interferon, MAF, IFNG, IFG, IFI|
|Description||Sharing 41% sequence identity with human Interferon gamma (hIFN--γ), mouse IFN gamma (mIFN--γ)is a macrophage-activating factor.The active form of IFN--γ is an antiparallel dimer that sets off IFN--γ/JAK/STAT pathway. IFN--γ signaling does diverse biological functions primarily related to host defense and immune regulation, including antiviral and antibacterial defense, apoptosis, inflammation, and innate and acquired immunity.While IFN--γ–induced inflammatory cascade summons a variety of immune-related cell types, such as macrophages, natural killer (NK) cells and cytotoxic T lymphocytes (CTLs), IFN--γ is also implicated in resistance to NK cell and CTL responses and in immune escape in avariety of cancers.
Recombinant mouse IFN gamma (rmIFN-γ) produced in E. coli is a non-glycosylated polypeptide chain of 134 amino acids. A fully biologically active molecule, rmIFN--γ has a molecular mass of 15 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary refolding and chromatographic techniques at GenScript.
|Biological Activity||ED50<0.15ng/ml, measured by cytotoxicity assay using WEHI-279 cells.|
MHGTVIESLE SLNNYFNSSG IDVEEKSLFL DIWRNWQKDG
DMKILQSQII SFYLRLFEVL KDNQAISNNI SVIESHLITT
FFSNSKAKKD AFMSIAKFEV NNPQVQRQAF NELIRVVHQL
|Measured Molecular Weight||15 kDa, observed by reducing SDS-PAGE.|
|Purity||> 95% as analyzed by reducing SDS-PAGE.|
|Formulation||Lyophilized after extensive dialysis against PBS.|
|Reconstitution||Reconstituted in ddH2O or PBS at 100 μg/ml.|
|Endotoxin Level||<1 EU/μg, determined by LAL method.|
|Storage||Lyophilized recombinant mouse IFN gamma (rmIFN-γ) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, rmIFN-γ should be stable up to 1week at 4°C or up to 2 months at -20°C.|
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