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Catalog Products » Recombinant Proteins » Cytokines » Interleukin-1 beta (IL-1β), Human

IL-1β, Human

Synonyms Catabolin, Lymphocyte-activating factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), Mononuclear Cell Factor (MCF), IL1F2, IL-1 beta.
Description Interleukin-1 beta (rhIL-1β) is a proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1 alpha and IL-1 beta binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1 beta is a secreted cytokine, IL-1 alpha is predominantly a cell-associated cytokine.

Recombinant human Interleukin-1 beta (rhIL-1β) produced in E. coli is a single non-glycosylated polypeptide chain containing 153 amino acids. A fully biologically active molecule, rhIL-1β has a molecular mass of 17.3kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
Species Human
Source E. coli
Biological Activity ED50< 10 pg/ml, measured by the dose-dependent stimulation of mouse D10.G4.1 helper T cells, corresponding to a specific activity of 1.0×108 IU/mg.
Sequence
APVRSLNCTL RDSQQKSLVM SGPYELKALH LQGQDMEQQV
VFSMSFVQGE ESNDKIPVAL GLKEKNLYLS CVLKDDKPTL
QLESVDPKNY PKKKMEKRFV FNKIEINNKL EFESAQFPNW
YISTSQAENM PVFLGGTKGG QDITDFTMQF VSS

Measured Molecular Weight 17.3 kDa, observed by reducing SDS-PAGE.
Purity > 95% by SDS-PAGE and HPLC analyses.
Formulation Lyophilized after extensive dialysis against PBS.
Reconstitution Reconstituted in ddH2O or PBS at 100 μg/ml.
Endotoxin Level <0.2 EU/μg, determined by LAL method.
Storage Lyophilized recombinant human Interleukin-1 beta (rhIL-1β) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, rhIL-1β should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.
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Cat. No. Z02922
Size
Price
50 ug $349.00
10 ug $95.00
1 mg $1750.00
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