Catalog Products » Recombinant Proteins » Growth Factors » Fibroblast Growth Factor-18 (FGF-18), Human

Fibrobalst Growth Factor-18(FGF-18), Human

Name: Fibrobalst Growth Factor-18(FGF-18), Human
SKU: Z02937-1
Rating: 5 (7 reviews)

Overview

Synonyms	zFGF5, FGFI
Description	Fibroblast growth factor 18 (FGF18) is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion. It has been shown in vitro that this protein is able to induce neurite outgrowth in PC12 cells. Studies of the similar proteins in mouse and chick suggested that this protein is a pleiotropic growth factor that stimulates proliferation in a number of tissues, most notably the liver and small intestine.
Source	Escherichia coli
Biological Activity	Fully biologically active when compared to standard. The ED50 determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 ng/ml, corresponding to a specific activity of >2.0 × 10⁶ IU/mg in the presence of 1 μg/ml of heparin.
Sequence	AEENVDFRIH VENQTRARDD VSRKQLRLYQ LYSRTSGKHI QVLGRRISAR GEDGDKYAQL LVETDTFGSQ VRIKGKETEF YLCMNRKGKL VGKPDGTSKE CVFIEKVLEN NYTALMSAKY SGWYVGFTKK GRPRKGPKTR ENQQDVHFMK RYPKGQPELQ KPFKYTTVTK RSRRIRPTHP A

Measured Molecular Weight	Approximately 21.1 kDa, a single non-glycosylated polypeptide chain containing 181amino acids.
Purity	>95% by SDS-PAGE and HPLC analyses.
Formulation	Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20°C. Further dilutions should be made in appropriate buffered solutions.
Endotoxin Level	Less than 1EU/μg of rHuFGF-18 as determined by LAL method.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Storage	This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.