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Catalog Products » Recombinant Proteins » Growth Factors » Epidermal Growth Factor (EGF), Mouse

EGF, Mouse

Synonyms Epidermal Growth Factor, Urogastrone, URG
Description Epidermal Growth Factor (EGF) was originally discovered in crude preparations of nerve growth factor prepared from mouse submaxillary glands as an activity that induced early eyelid opening, incisor eruption, hair growth inhibition, and stunting of growth when injected into newborn mice. EGF is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells. Additionally, EGF has been shown to inhibit gastric secretion, and to be involved in wound healing. EGF signals through a receptor known as c-erbB, which is a class I tyrosine kinase receptor. This receptor also binds with TGF-alpha and VGF (vaccinia virus growth factor).

Recombinant mouse Epidermal Growth Factor (rmEGF) produced in E. coli is a single non-glycosylated polypeptide chain containing 54 amino acids. A fully biologically active molecule, rmEGF is obtained by proprietary chromatographic techniques at GenScript with a molecular mass of 6.2kDa analyzed by reducing SDS-PAGE.
Species Mouse
Source E. coli
Biological Activity ED50 < 0.1ng/ml, measured by a cell proliferation assay using BALB/c 3T3 cells, corresponding to a specific activity of > 1.0× 107units/mg.
Sequence
MNSYPGCPSS YDGYCLNGGV CMHIESLDSY TCNCVIGYSG
DRCQTRDLRW WELR

Measured Molecular Weight 6.2 kDa, observed by reducing SDS-PAGE.
Purity > 95% by SDS-PAGE and HPLC analysis.
Formulation Lyophilized after extensive dialysis against PBS.
Reconstitution Reconstituted in ddH2O at 100 μg/ml.
Endotoxin Level < 0.2 EU/μg, determined by LAL method.
Storage Lyophilized recombinant mouse Epidermal Growth Factor (rmEGF) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, rmEGF should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.
Note For research use only

  • EGF, Mouse
  • EGF, Mouse

    ED₅₀< 0.1ng/ml, measured by a cell proliferation assay using BALB/c 3T3 cells, corresponding to a specific activity of > 1.0× 10⁷units/mg.




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Cat. No. Z02972
Size
Price
10 ug $15.00
50 ug $25.00
1 mg $125.00