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Catalog Products » Recombinant Proteins » Growth Factors » Granulocyte Colony-Stimulating Factor (G-CSF), Human

G-CSF, Human

G-CSF, Human

Biological Activity
G-CSF, Human (Cat. No. Z02974) stimulates cell proliferation of M-NFS-60 cells. The ED50 for this effect is typically 20-100pg/mL.

G-CSF, Human

2 μg of G-CSF, Human (Cat. No. Z02974) was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie Blue staining

Synonyms CSF-3, C17 or f33,
Description Granulocyte Colony-Stimulating Factor (G-CSF) contains internal disulfide bonds. Among the family of colony-stimulating factors, Granulocyte Colony Stimulating Factor (G-CSF) is the most potent inducer of terminal differentiation to granulocytes and macrophages of leukemic myeloid cell lines. The synthesis of Granulocyte Colony Stimulating Factor (G-CSF) can be induced by bacterial endotoxins, TNF, Interleukin-1 and GM-CSF. Prostaglandin E2 inhibits the synthesis of Granulocyte Colony Stimulating Factor (G-CSF). In epithelial, endothelial, and fibroblastic cells secretion of Granulocyte Colony Stimulating Factor (G-CSF) is induced by Interleukin-17.
Recombinant human Granulocyte Colony-Stimulating Factor (rhG-CSF) produced in E. coliis a single non-glycosylated polypeptide chain containing 175amino acids. A fully biologically active molecule, rhG-CSF is obtained by proprietary chromatographic techniques at GenScript, with an apparent molecular mass of 18.8kDa analyzed by reducing SDS-PAGE.
Species Human
Source E. coli
Biological Activity ED50 <0.1ng/ml, measured by a cell proliferation assay of M-NFS-60 cells, corresponding to a specific activity of >1.0× 107 IU/mg.
Sequence
MTPLGPASSL PQSFLLKCLE QVRKIQGDGA ALQEKLCATY
KLCHPEELVL LGHSLGIPWA PLSSCPSQAL QLAGCLSQLH
SGLFLYQGLL QALEGISPEL GPTLDTLQLD VADFATTIWQ
QMEELGMAPA LQPTQGAMPA FASAFQRRAG GVLVASHLQS
FLEVSYRVLR HLAQP

Measured Molecular Weight 18.8kDa, observed by reducing SDS-PAGE.
Purity > 95% by SDS-PAGE and HPLC analyses.
Formulation Lyophilized after extensive dialysis against 25mM Tris, pH8.0.
Reconstitution Reconstituted in ddH2O at 100 μg/ml.
Endotoxin Level <0.2 EU/μg, determined by LAL method.
Storage Lyophilized recombinant human Granulocyte Colony-Stimulating Factor (rhG-CSF) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, rhG-CSF should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.
Note For research use only

  • G-CSF, Human
  • G-CSF, Human

    Biological Activity
    G-CSF, Human (Cat. No. Z02974) stimulates cell proliferation of M-NFS-60 cells. The ED50 for this effect is typically 20-100pg/mL.

  • G-CSF, Human
  • G-CSF, Human

    2 μg of G-CSF, Human (Cat. No. Z02974) was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie Blue staining


Xie MW, .et al Marrow-Derived Stromal Cell Delivery on Fibrin Microbeads Can Correct Radiation-Induced Wound-Healing Deficits. J Invest Dermatol. 2013 Feb;133(2):553-61.

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Cat. No. Z02974
Size
Price
10 ug $75.00
50 ug $250.00
1 mg $1680.00