|Synonyms||HBGF-4; HST; HST-1; HSTF1; K-FGF; KFGF.|
|Description||Fibroblast Growth Factor-4 (FGF-4) also known as K-FGF is a heparin-binding growth factor of the FGF family.It was identified by its oncogenic transforming activity. FGF-4 and FGF-3 are located closely on chromosome 11. FGF-4 and its receptors (FGF R1c, 2c, 3c and 4) play an important role in the regulation of embryonic development, cell proliferation, and cell differentiation. FGF-4 is required for normal limb and cardiac valve development during embryogenesis.
Recombinant human Fibroblast Growth Factor-4 (rhFGF-4) produced in E. coli is a single non-glycosylated polypeptide chain containing 177 amino acids. A fully biologically active molecule obtained by proprietary chromatographic techniques at GenScript, rhFGF-4 has a molecular mass of 19.4kDa as analyzed by reducing SDS-PAGE.
|Biological Activity||ED50<0.5ng/ml, measured in a cell proliferation assay using 3T3 cells, corresponding to a specific activity of >2.0× 106 units/mg.|
MAPTAPNGTL EAELERRWES LVALSLARLP VAAQPKEAAV
QSGAGDYLLG IKRLRRLYCN VGIGFHLQAL PDGRIGGAHA
DTRDSLLELS PVERGVVSIF GVASRFFVAM SSKGKLYGSP
FFTDECTFKE ILLPNNYNAY ESYKYPGMFI ALSKNGKTKK
|Measured Molecular Weight||19.4kDa, observed by reducing SDS-PAGE.|
|Purity||> 95% by SDS-PAGE and HPLC analyses.|
|Formulation||Lyophilized after extensive dialysis against 50mM HEPES, 750mM NaCl, pH7.5.|
|Reconstitution||Reconstituted in ddH2O at 100 μg/ml.|
|Endotoxin Level||<0.2 EU/μg, determined by LAL method.|
|Storage||Lyophilized recombinant human Fibroblast Growth Factor-4 (rhFGF-4) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, rhFGF-4 should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.|
|Note||For research use only|
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