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Catalog Products » Recombinant Proteins » Cytokines » Interleukin-1 beta (IL-1β), Mouse

IL-1β, Mouse

Synonyms Catabolin, Lymphocyte-activating factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), Mononuclear Cell Factor (MCF)
Description Interleukin-1 Beta (IL-1β) is a proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1 alpha and IL-1 beta binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1 beta is a secreted cytokine, IL-1 alpha is predominantly a cell-associated cytokine.
Recombinant mouse Interleukin-1 Beta(rmIL-1β) produced in E. coli is a single non-glycosylated polypeptide chain containing 153 amino acids. A fully biologically active molecule, rmIL-1β is obtained by proprietary chromatographic techniques at GenScript, with an apparent molecular weight of 17.5kDa analyzed by reducing SDS-PAGE.
Species Mouse
Source E. coli
Biological Activity ED50< 10 pg/ml, measured by the dose-dependent stimulation of mouse D10.G4.1 helper T cells, corresponding to a specific activity of 1.0×108 IU/mg.
Sequence
MVPIRQLHYR LRDEQQKSLV LSDPYELKAL HLNGQNINQQ
VIFSMSFVQG EPSNDKIPVA LGLKGKNLYL SCVMKDGTPT
LQLESVDPKQ YPKKKMEKRF VFNKIEVKSK VEFESAEFPN
WYISTSQAEH KPVFLGNNSG QDIIDFTMES VSS

Measured Molecular Weight 17.5kDa, observed by reducing SDS-PAGE.
Purity > 95% by SDS-PAGE and HPLC analyses.
Formulation Lyophilized after extensive dialysis against PBS.
Reconstitution Reconstituted in ddH2O or PBS at 100 μg/ml.
Endotoxin Level <0.2 EU/μg, determined by LAL method.
Storage Lyophilized recombinant mouseInterleukin-1 Beta(rmIL-1β) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, rmIL-1β should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.
Note For research use only

  • IL-1β, Mouse
  • IL-1β, Mouse

    Biological Activity
    IL-1beta, Mouse (Cat. No. Z02985) stimulates cell proliferation of D10S cells. The ED50 for this effect is less than 2pg/mL.


Hua Zhang,. et al.Ex vivo spinal cord slice model of neuromyelitis optica reveals novel immunopathogenic mechanisms.Ann Neurol.2011 Dec;70(6):943-54

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Cat. No. Z02985
Size
Price
50 ug $349.00
10 ug $95.00
1 mg $1750.00
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