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Catalog Products » Recombinant Proteins » Cytokines » Interleukin-1 beta (IL-1β), Mouse(CHO-expressed)

IL-1β, Mouse(CHO-expressed)

Synonyms Catabolin, Lymphocyte-activating factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), Mononuclear Cell Factor (MCF)
Description Interleukin-1 (IL-1) is a family of cytokines that play a central role in the regulation of immune and inflammatory responses to infections or sterile insults. IL-1α and IL-1β are the first two members discovered in this family, which are the products of distinct genes recognizing the same cell surface receptors. IL-1α and IL-1β are structurally related polypeptides that show approximately 25% homology at the amino acid level. Both proteins are produced by a wide variety of cells in response to stimuli such as those produced by inflammatory agents, infections, or microbial endotoxins. The proteins are synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17.5 kDa. The specific protease responsible for the processing of IL-1β is interleukin 1β-converting enzyme (ICE)/caspase-1. Mature human and mouse IL-1β share approximately 75% amino acid sequence identity where human IL-1β has been found to be active on murine cell lines.

GenScript Interleukin (IL)-1β, murine, produced in CHO cells, is a non-glycosylated polypeptide chain containing 152 amino acids and having a molecular mass of 17,400 Da.
Species Mouse
Source CHO
Specific Activity ED50 < 10 pg/ml, measured in a cell proliferation assay using D10S cells, corresponding to a specific activity of > 1×108 units/mg.
Sequence
VPIRQLHYRL RDEQQKSLVL SDPYELKALH LNGQNINQQV
IFSMSFVQGE PSNDKIPVAL GLKGKNLYLS CVMKDGTPTL
QLESVDPKQY PKKKMEKRFV FNKIEVKSKV EFESAEFPNW
YISTSQAEHK PVFLGNNSGQ DIIDFTMESV SS

Measured Molecular Weight 17.4 kDa, observed by non-reducing SDS-PAGE.
Purity > 95% as analyzed by SDS-PAGE and HPLC.
Formulation Lyophilized after extensive dialysis against PBS.
Reconstitution Reconstituted in ddH2O or PBS at 100 μg/ml.
Endotoxin Level <0.2 EU/μg, determined by LAL method.
Storage Lyophilized recombinant Murine Interleukin 1 beta (IL-1 beta) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rmIL-1beta should be stable up to 1 week at 4°C or up to 2 months at -20°C.
Note For research use only




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Cat. No. Z02988
Size
Price
50 ug $249.00
10 ug $75.00
1 mg $1680.00
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