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Catalog Products » Recombinant Proteins » Growth Factors » Fibroblast Growth Factor-8 (FGF-8), Human

FGF-8, Human

Synonyms FGF-8b, AIGF, HBGF
Description Fibroblast Growth Factor-8 (FGF-8) is a heparin-binding growth factor of the FGF family. There are 4 known forms of FGF8 produced by alternative splicing: FGF8a, FGF-8b, FGF-8e and FGF-8f. The human and mouse FGF8b are identical of aa sequences. FGF-8 plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. FGF-8 is required for normal brain, eye, ear and limb development during embryogenesis. It is also required for normal development of the gonadotropin- releasing hormone (GnRH) neuronal system.
Recombinant human Fibroblast Growth Factor-8 (rhFGF-8) produced in E. coli is a single non-glycosylated polypeptide chain containing 194 amino acids. A fully biologically active molecule, rhFGF-8 has a molecular mass of 22.5kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
Species Human
Source E. coli
Biological Activity ED50 < 5.0 ng/ml, measured by a cell proliferation assay using 3T3 cells in the presence of 1μg/ml of heparin, corresponding to a specific activity of > 2.0× 105 units/mg.
Sequence
MQVTVQSSPN FTQHVREQSL VTDQLSRRLI RTYQLYSRTS
GKHVQVLANK RINAMAEDGD PFAKLIVETD TFGSRVRVRG
AETGLYICMN KKGKLIAKSN GKGKDCVFTE IVLENNYTAL
QNAKYEGWYM AFTRKGRPRK GSKTRQHQRE VHFMKRLPRG
HHTTEQSLRF EFLNYPPFTR SLRGSQRTWA PEPR

Measured Molecular Weight 22.5kDa, observed by reducing SDS-PAGE.
Purity > 95% by SDS-PAGE and HPLC analyses.
Formulation Lyophilized after extensive dialysis against PBS.
Reconstitution Reconstituted in ddH2O at 100 μg/ml.
Endotoxin Level < 0.2 EU/μg, determined by LAL method.
Storage Lyophilized recombinant human Fibroblast Growth Factor-8 (rhFGF-8) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, rhFGF-8 should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.
Note For research use only

  • FGF-8, Human
  • FGF-8, Human

    ED₅₀< 5.0 ng/ml, measured by a cell proliferation assay using 3T3 cells in the presence of 1μg/ml of heparin, corresponding to a specific activity of > 2.0× 10⁵ units/mg.




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Cat. No. Z02989
Size
Price
10 ug $65.00
50 ug $159.00
1 mg $1500.00