|Synonyms||FGF-8b, AIGF, HBGF|
|Description||Fibroblast Growth Factor-8 (FGF-8) is a heparin-binding growth factor of the FGF family. There are 4 known forms of FGF8 produced by alternative splicing: FGF8a, FGF-8b, FGF-8e and FGF-8f. The human and mouse FGF8b are identical of aa sequences. FGF-8 plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. FGF-8 is required for normal brain, eye, ear and limb development during embryogenesis. It is also required for normal development of the gonadotropin- releasing hormone (GnRH) neuronal system.
Recombinant human Fibroblast Growth Factor-8 (rhFGF-8) produced in E. coli is a single non-glycosylated polypeptide chain containing 194 amino acids. A fully biologically active molecule, rhFGF-8 has a molecular mass of 22.5kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
|Biological Activity||ED50 < 5.0 ng/ml, measured by a cell proliferation assay using 3T3 cells in the presence of 1μg/ml of heparin, corresponding to a specific activity of > 2.0× 105 units/mg.|
MQVTVQSSPN FTQHVREQSL VTDQLSRRLI RTYQLYSRTS
GKHVQVLANK RINAMAEDGD PFAKLIVETD TFGSRVRVRG
AETGLYICMN KKGKLIAKSN GKGKDCVFTE IVLENNYTAL
QNAKYEGWYM AFTRKGRPRK GSKTRQHQRE VHFMKRLPRG
HHTTEQSLRF EFLNYPPFTR SLRGSQRTWA PEPR
|Measured Molecular Weight||22.5kDa, observed by reducing SDS-PAGE.|
|Purity||> 95% by SDS-PAGE and HPLC analyses.|
|Formulation||Lyophilized after extensive dialysis against PBS.|
|Reconstitution||Reconstituted in ddH2O at 100 μg/ml.|
|Endotoxin Level||< 0.2 EU/μg, determined by LAL method.|
|Storage||Lyophilized recombinant human Fibroblast Growth Factor-8 (rhFGF-8) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, rhFGF-8 should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.|
|Note||For research use only|
For more documents, please visit "Technical Support".