|Synonyms||MCGF (Mast cell growth factor), Multi-CSF, HCGF, P-cell stimulation factor|
|Description||Interleukin-3 (IL-3) is a hematopoietic growth factor that promotes the survival, differentiation and proliferation of committed progenitor cells of the megakaryocyte, granulocyte-macrophage, erythroid, eosinophil, basophil and mast cell lineages. Produced by T cells, mast cells and eosinophils, IL-3 enhances thrombopoieses, phagocytosis, and antibody-mediated cellular cytotoxicity. Its ability to activate monocytes suggests that IL-3 may have additional immunoregulatory roles. Many of the IL-3 activities depend upon co-stimulation with other cytokines. IL-3 is species-specific, variably glycosylated cytokine.
Recombinant human interleukin 3 expressed in CHO cells is glycosylated protein with molecular weight range from 17 to 30 kDa shown in non-reducing SDS-PAGE.
|Biological Activity||ED50 < 0.1 ng/ml, measured in a cell proliferation assay using TF-1 cells, corresponding to a specific activity of > 1×107 units/mg.|
APMTQTTPLK TSWVNCSNMI DEIITHLKQP PLPLLDFNNL
NGEDQDILME NNLRRPNLEA FNRAVKSLQN ASAIESILKN
LLPCLPLATA APTRHPIHIK DGDWNEFRRK LTFYLKTLEN
|Measured Molecular Weight||17-30 kDa, observed by non-reducing SDS-PAGE.|
|Purity||> 95% as analyzed by SDS-PAGE and HPLC.|
|Formulation||Lyophilized after extensive dialysis against PBS.|
|Reconstitution||Reconstituted in ddH2O or PBS at 100 μg/ml.|
|Endotoxin Level||< 0.2 EU/μg, determined by LAL method.|
|Storage||Lyophilized recombinant human Interlerkin 3 (IL-3) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, rhIL-3 should be stable up to 1 week at 4°C or up to 2 months at -20°C.|
|Note||For research use only|
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