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Catalog Products » Recombinant Proteins » Cytokines » Interleukin-4 (IL-4), Mouse

IL-4, Mouse

Synonyms BCGF, BCDF, B cell stimulating factor (BSF-1)
Description Interleukin-4 (IL-4) is a pleiotropic cytokine regulates diverse T and B cell responses including cell proliferation, survival, and gene expression. It has important effects on the growth and differentiation of different immunologically competent cells. Interleukin-4 is produced by mast cells, T cells, and bone marrow stromal cells[1]. IL-4 regulates the differentiation of native CD4+ T cells (Th0 cells) into helper Th2 cells, and regulates the immunoglobulin class switching to the IgG1 and IgE isotypes. IL-4 has numerous important biological functions including stimulating B-cells activation, T-cell proliferation and CD4+ T-cells differentiation to Th2 cells. It is a key regulator in hormone control and adaptive immunity[2]. IL-4 also plays a major role in inflammation response and wound repair via activation of macrophage into M2 cells[3]. IL-4 is stabilized by three disulphide bonds forming a compact globular protein structure[4]. Four alpha-helix bundle with left-handed twist is dominated half of the protein structure with 2 overhand connections and fall into a 2-stranded anti-parallel beta sheet[5].
Species Mouse
Source CHO
Biological Activity ED50 < 2 ng/ml, measured in a cell proliferation assay using murine HT-2 cells, corresponding to a specific activity of >5 x 105 units/mg.
Sequence
HIHGCDKNHL REIIGILNEV TGEGTPCTEM DVPNVLTATK
NTTESELVCR ASKVLRIFYL KHGKTPCLKK NSSVLMELQR
LFRAFRCLDS SISCTMNESK STSLKDFLES LKSIMQMDYS

Measured Molecular Weight 15 kDa, observed by reducing SDS-PAGE.
Purity > 95% as analyzed by SDS-PAGE and HPLC.
Formulation Lyophilized after extensive dialysis against PBS.
Reconstitution Reconstituted in ddH2O or PBS at 100 μg/ml.
Endotoxin Level < 0.2 EU/μg, determined by LAL method.
Storage Lyophilized recombinant Murine Interleukin 4 (IL-4) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, rmIL-4 should be stable up to 1 week at 4°C or up to 2 months at -20°C.
Note For research use only


1. Nguyen, T., et al. (2011) Immunol Rev.,242:258-271

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Cat. No. Z02996
Size
Price
50 ug $139.00
10 ug $75.00
1 mg $1200.00
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