|Synonyms||Stem Cell Factor, c-Kit Ligand, Mast Cell Growth Factor (MGF), Steel Factor|
|Description||Stem Cell Factor (SCF) is a hematopoietic growth factor that binds to the c-Kit receptor. SCF exerts its activity during the early stages of hematopoiesis. SCF stimulates the proliferation of myeloid, erythroid, and lymphoid progenitors in bone marrow cultures and has been shown to act synergistically with colony stimulating factors.
Recombinant mouse Stem Cell Factor (rmSCF) produced in P. pastoris is a single glycosylated polypeptide chain containing 165 amino acids. A fully biologically active molecule, rmSCF has a molecular mass of 18.4kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
|Biological Activity||ED50 < 10.0 ng/ml, measured by a cell proliferation assay using human TF-1 cells, corresponding to a specific activity of > 1.0× 105 units/mg.|
MKEICGNPVT DNVKDITKLV ANLPNDYMIT LNYVAGMDVL
PSHCWLRDMV IQLSLSLTTL LDKFSNISEG LSNYSIIDKL
GKIVDDLVLC MEENAPKNIK ESPKRPETRS FTPEEFFSIF
NRSIDAFKDF MVASDTSDCV LSSTLGPEKD SRVSVTKPFM
|Measured Molecular Weight||18.4kDa, observed by reducing SDS-PAGE.|
|Purity||> 95% by SDS-PAGE and HPLC analyses.|
|Formulation||Lyophilized after extensive dialysis against 50mM Tris, pH8.0.|
|Reconstitution||Reconstituted in ddH2O at 100 μg/ml.|
|Endotoxin Level||< 0.2 EU/μg, determined by LAL method.|
|Storage||Lyophilized recombinant mouse Stem Cell Factor (rmSCF) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, rmSCF should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.|
|Note||For research use only|
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