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Catalog Products » Recombinant Proteins » Cytokines » Tumor Necrosis Factor-alpha (TNF-α), Rat

TNF-α, Rat

Synonyms Tumor Necrosis Factor, TNFSF2, Cachectin, Differentiation-inducing factor (DIF), Necrosin, Cytotoxin
Description Tumor Necrosis Factor-Alpha (TNF-Alpha) also known as Cachectin and TNFSF1A, plays a major role in growth regulation, differentiation, inflammation, viral replication, tumorigenesis, and autoimmune diseases; and in viral, bacterial, fungal, and parasitic infections. Besides inducing hemorrhagic necrosis of tumors, TNF has been found to be involved in tumorigenesis, tumor metastasis, viral replication, septic shock, fever, inflammation, and autoimmune diseases including Crohn's disease, and rheumatoid arthritis as well as graft-versus-host disease.

Recombinant rat Tumor Necrosis Factor-Alpha (rrTNF-Alpha) produced in P. pastoris is a glycosylated polypeptide chain containing 157 amino acids. A fully biologically active molecule, rrTNF-Alpha has a molecular mass of 17.4kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
Species Rat
Source P. pastoris
Biological Activity ED50 < 50 pg/ml, measured in a cytotoxicity assay using mouse L-929 cells in the presence of actinomycin D, corresponding to a specific activity of > 2.0× 107 units/mg.
Sequence
MLRSSSQNSS DKPVAHVVAN HQAEEQLEWL SQRANALLAN
GMDLKDNQLV VPADGLYLIY SQVLFKGQGC PDYVLLTHTV
SRFAISYQEK VSLLSAIKSP CPKDTPEGAE LKPWYEPMYL
GGVFQLEKGD LLSAEVNLPK YLDITESGQV YFGVIAL

Measured Molecular Weight 17.4kDa, observed by reducing SDS-PAGE.
Purity > 95% by SDS-PAGE and HPLC analyses.
Formulation Lyophilized after extensive dialysis against PBS.
Reconstitution Reconstituted in ddH2O at 100 μg/ml.
Endotoxin Level < 0.2 EU/μg, determined by LAL method.
Storage Lyophilized recombinant rat Tumor Necrosis Factor-Alpha (rrTNF-Alpha) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, rrTNF-Alpha should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.
Note For research use only




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Cat. No. Z02999
Size
Price
50 ug $138.00
10 ug $49.00
1 mg $975.00
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