|Synonyms||Tumor Necrosis Factor, TNFSF2, Cachectin, Differentiation-inducing factor (DIF), Necrosin, Cytotoxin|
|Description||Tumor Necrosis Factor-Alpha (TNF-Alpha) also known as Cachectin and TNFSF1A, plays a major role in growth regulation, differentiation, inflammation, viral replication, tumorigenesis, and autoimmune diseases; and in viral, bacterial, fungal, and parasitic infections. Besides inducing hemorrhagic necrosis of tumors, TNF has been found to be involved in tumorigenesis, tumor metastasis, viral replication, septic shock, fever, inflammation, and autoimmune diseases including Crohn's disease, and rheumatoid arthritis as well as graft-versus-host disease.
Recombinant rat Tumor Necrosis Factor-Alpha (rrTNF-Alpha) produced in P. pastoris is a glycosylated polypeptide chain containing 157 amino acids. A fully biologically active molecule, rrTNF-Alpha has a molecular mass of 17.4kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
|Specific Activity||ED50 < 50 pg/ml, measured in a cytotoxicity assay using mouse L-929 cells in the presence of actinomycin D, corresponding to a specific activity of > 2.0× 107 units/mg.|
MLRSSSQNSS DKPVAHVVAN HQAEEQLEWL SQRANALLAN
GMDLKDNQLV VPADGLYLIY SQVLFKGQGC PDYVLLTHTV
SRFAISYQEK VSLLSAIKSP CPKDTPEGAE LKPWYEPMYL
GGVFQLEKGD LLSAEVNLPK YLDITESGQV YFGVIAL
|Measured Molecular Weight||17.4kDa, observed by reducing SDS-PAGE.|
|Purity||> 95% by SDS-PAGE and HPLC analyses.|
|Formulation||Lyophilized after extensive dialysis against PBS.|
|Reconstitution||Reconstituted in ddH2O at 100 μg/ml.|
|Endotoxin Level||< 0.2 EU/μg, determined by LAL method.|
|Storage||Lyophilized recombinant rat Tumor Necrosis Factor-Alpha (rrTNF-Alpha) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rrTNF-Alpha should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.|
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