|Synonyms||Neurotrophin-4, Neurotrophic 4/5 (NT-4/NT-5)|
|Description||Neurotrophin-4 (NT-4), also known as NT-5, is a neurotrophic factor structurally related to β-NGF, BDNF, and NT-3. Human NT-4 shares 48 - 52% aa sequence identity with human β-NGF, BDNF, and NT-3.Neurotrophins have six conserved cysteine residues that are involved in the formation of three disulfide bonds. NT-4 is expressed highest levels in prostate, lower levels in thymus, placenta, and skeletal muscle. NT-4 binds and induces receptor dimerization and activation of TrkB. NT-4 can signal through TrkB receptors and promotes the survival of peripheral sensory sympathetic neurons.
Recombinant human Neurotrophin-4 (rhNT-4) produced in E.coli is a noncovalently linked homodimer containing two non-glycosylated polypeptide chains of 131 amino acids. A fully biologically active molecule, rhNT-4 has a molecular mass of 28.1kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
|Biological Activity||ED50 < 5.0 μg/ml, measured by a cell proliferation assay using C6 cells, corresponding to a specific activity of > 2.0× 102 units/mg.|
MGVSETAPAS RRGELAVCDA VSGWVTDRRT AVDLRGREVE
VLGEVPAAGG SPLRQYFFET RCKADNAEEG GPGAGGGGCR
GVDRRHWVSE CKAKQSYVRA LTADAQGRVG WRWIRIDTAC
|Measured Molecular Weight||28.1 kDa, a noncovalently linked homodimer,of two 14.0 kDa polypeptide monomers.|
|Purity||> 95% by SDS-PAGE and HPLC analyses.|
|Formulation||Lyophilized after extensive dialysis against 50mM acetic acid.|
|Reconstitution||Reconstituted in 50mM acetic acid or ddH2O at 50 μg/ml.|
|Endotoxin Level||< 0.3 EU/μg, determined by LAL method.|
|Storage||Lyophilized recombinant human Neurotrophin-4 (rhNT-4) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, rhNT-4 should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.|
|Note||For research use only|
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