• English
  • Sign In
  • Contact Us
×
Catalog Products » Recombinant Proteins » Cytokines » Interferon-alpha 2b (IFN-α 2b), Human

IFN-α 2b, Human

IFN-α 2b, Human

Biological Activity
IFN-alpha 2b, Human (Cat. No. Z03002) induced cytotoxicity of R&D TF-1 cells. The ED50 for this effect is typically 20-50pg/mL.

IFN-α 2b, Human

2 μg of IFN-α 2b, Human (Cat. No. Z03002) was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie Blue staining.2 μg of IFN-α 2b, Human (Cat. No. Z03002) was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie Blue staining.

Synonyms Interferon alpha 2b, IFNA, INFA2, MGC125764, MGC125765
Description Interferon-Alpha 2b (IFN-Alpha 2b) produced by leukocytes is a member of Interferon family. IFN-alpha is mainly involved in innate immune response against a broad range of viral infections. IFN-alpha 2 has three acid stable forms (a,b,c) signaling through IFNAR2. IFN-alpha 2b shares 99.4% aa sequence identity with both IFN-alpha 2a and 2c. IFN-alpha contains four highly conserved cysteine residues which form two disulfide bonds, one of which is necessary for biological activity.

Recombinant human Interferon-Alpha 2b (rhIFN-Alpha 2b) produced in E.coli is a single non-glycosylated polypeptide chain containing 165 amino acids. A fully biologically active molecule, rhIFN-Alpha 2b has a molecular mass of 19.2kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
Species Human
Source E. coli
Biological Activity ED50 < 0.05 ng/ml, measured by a cytotoxicity assay using TF-1 Cells, corresponding to a specific activity of > 2.0× 107 units/mg.
Sequence
CDLPQTHSLG SRRTLMLLAQ MRRISLFSCL KDRHDFGFPQ
EEFGNQFQKA ETIPVLHEMI QQIFNLFSTK DSSAAWDETL
LDKFYTELYQ QLNDLEACVI QGVGVTETPL MKEDSILAVR
KYFQRITLYL KEKKYSPCAW EVVRAEIMRS FSLSTNLQES
LRSKE

Measured Molecular Weight 19.2kDa, observed by reducing SDS-PAGE.
Purity > 95% by SDS-PAGE and HPLC analyses.
Formulation Lyophilized after extensive dialysis against PBS.
Reconstitution Reconstituted in ddH2O at 100 μg/ml.
Endotoxin Level < 0.2 EU/μg, determined by LAL method.
Storage Lyophilized recombinant human Interferon-Alpha 2b (rhIFN-Alpha 2b) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, rhIFN-Alpha 2b should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.
Note For research use only

  • IFN-α 2b, Human
  • IFN-α 2b, Human

    Biological Activity
    IFN-alpha 2b, Human (Cat. No. Z03002) induced cytotoxicity of R&D TF-1 cells. The ED50 for this effect is typically 20-50pg/mL.

  • IFN-α 2b, Human
  • IFN-α 2b, Human

    2 μg of IFN-α 2b, Human (Cat. No. Z03002) was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie Blue staining.2 μg of IFN-α 2b, Human (Cat. No. Z03002) was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie Blue staining.


Perales C, .et alResponse of hepatitis C virus to long-term passage in the presence of interferon-α. Multiple mutations and a common phenotype.J. Virol. 2013 Jul;87(13):7593-607

You can visit "Citations Database" for more citations.






For more documents, please visit "Technical Support".

Cat. No. Z03002
Size
Price
50 ug $49.00
10 ug $25.00
1 mg $325.00