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Catalog Products » Recombinant Proteins » Cytokines » Interferon-alpha 2a (IFN-α 2a), Human

IFN-α 2a, Human

IFN-α 2a, Human

Biological Activity
IFN-alpha 2a, Human (Cat. No. Z03003) induced cytotoxicity of R&D TF-1 cells. The ED50 for this effect is less than 0.1ng/mL.

IFN-α 2a, Human

2 μg of IFN-α 2a, Human (Cat. No. Z03003) was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie Blue staining.

Synonyms Leukocyte interferon, B cell interferon, Type I interferon, IFNA2, IFN-a 2a.
Description Interferon-Alpha 2a (IFN-Alpha 2a), Human produced by leukocytes is a member of Interferon family. IFN-alpha is mainly involved in innate immune response against a broad range of viral infections. IFN-alpha 2 has three acid stable forms (a,b,c) signaling through IFNAR2. IFN-alpha 2a shares 99.4% , 98.8% aa sequence identity with IFN-alpha 2b and 2c respectively. IFN-alpha contains four highly conserved cysteine residues which form two disulfide bonds, one of which is necessary for biological activity.

Recombinant Interferon-Alpha 2a (IFN-Alpha 2a), Human produced in E. coli is a single non-glycosylated polypeptide chain containing 165 amino acids. A fully biologically active molecule, rhIFN-Alpha has a molecular mass of 19.2kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
Species Human
Source E. coli
Specific Activity ED50 < 0.1 ng/ml, measured by a cytotoxicity assay using TF-1 Cells, corresponding to a specific activity of > 1.0× 107 units/mg.
Sequence
CDLPQTHSLG SRRTLMLLAQ MRKISLFSCL KDRHDFGFPQ
EEFGNQFQKA ETIPVLHEMI QQIFNLFSTK DSSAAWDETL
LDKFYTELYQ QLNDLEACVI QGVGVTETPL MKEDSILAVR
KYFQRITLYL KEKKYSPCAW EVVRAEIMRS FSLSTNLQES
LRSKE

Measured Molecular Weight 19.2kDa, observed by reducing SDS-PAGE.
Purity > 95% by SDS-PAGE and HPLC analyses.
Formulation Lyophilized after extensive dialysis against PBS.
Reconstitution Reconstituted in ddH2O at 100 μg/ml.
Endotoxin Level < 0.2 EU/μg, determined by LAL method.
Storage Lyophilized recombinant human Interferon-Alpha 2a (rhIFN-Alpha 2a) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhIFN-Alpha 2a should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.
Note For research use only

  • IFN-α 2a, Human
  • IFN-α 2a, Human

    Biological Activity
    IFN-alpha 2a, Human (Cat. No. Z03003) induced cytotoxicity of R&D TF-1 cells. The ED50 for this effect is less than 0.1ng/mL.

  • IFN-α 2a, Human
  • IFN-α 2a, Human

    2 μg of IFN-α 2a, Human (Cat. No. Z03003) was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie Blue staining.


Da Silva-Ferrada E,et al.Analysis of SUMOylated proteins using SUMO-traps.Sci Rep.2013 3:1690

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Cat. No. Z03003
Size
Price
50 ug $49.00
10 ug $25.00
1 mg $325.00
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