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Catalog Products » Recombinant Proteins » Growth Factors » Sonic Hedgehog (Shh), Mouse(CHO-expressed)

Shh, Mouse(CHO-expressed)

Synonyms SHH, HHG-1
Description Members of the Hedgehog (Hh) family are highly conserved proteins which are widely represented throughout the animal kingdom. The three known mammalian Hh proteins, Sonic (Shh), Desert (Dhh) and Indian (Ihh) are structurally related and share a high degree of amino-acid sequence identity (e.g., Shh and Ihh are 93% identical). The biologically active form of Hh molecules is obtained by autocatalytic cleavage of their precursor proteins and corresponds to approximately the N-terminal one half of the precursor molecule. Although Hh proteins have unique expression patterns and distinct biological roles within their respective regions of secretion, they use the same signaling pathway and can substitute for each other in experimental systems. Recombinant E. coli derived Human Sonic HedgeHog is a 20.0 kDa protein consisting of 176 amino acid residues, including an N-terminal Ile-Val-Ile sequence substituted for the natural occurring chemically modified Cys residue.
Species Mouse
Source CHO
Biological Activity ED50 < 1 µg/ml, measured by its ability to induce alkaline phosphatase production by CCL-226 cells, corresponding to a specific activity of >1 x 103 units/mg.
Sequence
CGPGRGFGKR RHPKKLTPLA YKQFIPNVAE KTLGASGRYE
GKITRNSERF KELTPNYNPD IIFKDEENTG ADRLMTQRCK
DKLNALAISV MNQWPGVKLR VTEGWDEDGH HSEESLHYEG
RAVDITTSDR DRSKYGMLAR LAVEAGFDWV YYESKAHIHC
SVKAENSVAA KSGG

Measured Molecular Weight 20 kDa, observed by non-reducing SDS-PAGE.
Purity > 95% as analyzed by SDS-PAGE and HPLC.
Formulation Lyophilized after extensive dialysis against PBS.
Reconstitution Reconstituted in ddH2O or PBS at 100 μg/ml.
Endotoxin Level < 0.2 EU/μg, determined by LAL method.
Storage Lyophilized recombinant Murine Sonic Hedgehog (SHH) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, rmSHH should be stable up to 1 week at 4°C or up to 2 months at -20°C.
Note For research use only

  • Shh, Mouse(CHO-expressed)
  • Shh, Mouse(CHO-expressed)

    ED₅₀< 1 µg/ml, measured by its ability to induce alkaline phosphatase production by CCL-226 cells, corresponding to a specific activity of >1 x 10³ units/mg.


Cat. No.
Product Name
Price
Z03067-10
Shh (C24II), Human - 10 ug
$65.00
Z03069-10
Shh (C24IVI), Human - 10 ug
$65.00
Z03050-10
Shh (C25II), Mouse - 10 ug
$65.00
Z02910-25
Shh, Human - 25 ug
$159.00
Z02990-1
Shh, Mouse - 1 mg
$1200.00
 



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Cat. No. Z03008
Size
Price
10 ug $65.00
50 ug $159.00
1 mg $1200.00