|Synonyms||Fibroblast Growth Factor-18, zFGF5, FGFI|
|Description||Fibroblast Growth Factor-18 (FGF-18) is a heparin-binding growth factor that is a member of the FGF family. FGF-18 signals through FGFR 1c, 2c, 3c, and 4. FGF-18 plays an important role in the regulation of cell proliferation, cell differentiation and cell migration. FGF-18 is required for normal ossification and bone development. It can also stimulate hepatic and intestinal proliferation.
Recombinant human Fibroblast Growth Factor-18 (rhFGF-18) produced in E. coli is a single non-glycosylated polypeptide chain containing 174 amino acids. A fully biologically active molecule, rhFGF-18 has a molecular mass of 20.3 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
|Biological Activity||ED50 < 10 ng/ml, measured by a cell proliferation assay using 3T3 Cells, corresponding to a specific activity of > 1.0× 105units/mg.|
MAEENVDFRI HVENQTRARD DVSRKQLRLY QLYSRTSGKH
IQVLGRRISA RGEDGDKYAQ LLVETDTFGS QVRIKGKETE
FYLCMNRKGK LVGKPDGTSK ECVFIEKVLE NNYTALMSAK
YSGWYVGFTK KGRPRKGPKT RENQQDVHFM KRYPKGQPEL
|Measured Molecular Weight||20.3 kDa, observed by reducing SDS-PAGE.|
|Purity||> 95% by SDS-PAGE and HPLC analyses.|
|Formulation||Lyophilized after extensive dialysis against PBS.|
|Reconstitution||Reconstituted in ddH2O at 100 μg/ml.|
|Endotoxin Level||< 0.2 EU/μg, determined by LAL method.|
|Storage||Lyophilized recombinant human Fibroblast Growth Factor-18 (rhFGF-18) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, rhFGF-18 should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.|
|Note||For research use only|
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