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Catalog Products » Recombinant Proteins » Cytokines » Interleukin-1 beta (IL-1β), Rat

Interleukin-1 beta (IL-1β), Rat



Synonyms IL1F2, IL1B, Catabolin, Leukocyte Endogenous Mediator (LEM), Mononuclear Cell Factor (MCF)
Description Interleukin-1 Beta (IL-1 Beta) is a proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1 alpha and IL-1 beta bind to the same receptor and have similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1 beta is a secreted cytokine, IL-1 alpha is predominantly a cell-associated cytokine.

Recombinant rat Interleukin-1 Beta (rat IL-1 Beta) produced in E. coli is a single non-glycosylated polypeptide chain containing 152 amino acids. A fully biologically active molecule, recombinant rat IL-1 Beta protein has a molecular mass of 17.3kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
Accession No Q63264
Species Rat
Source E. coli
Specific Activity ED50 < 10 pg/ml, measured by a cell proliferation assay using mouse D10S cells, corresponding to a specific activity of > 1.0× 108 units/mg.
Sequence
VPIRQLHCRL RDEQQKCLVL SDPCELKALH LNGQNISQQV
VFSMSFVQGE TSNDKIPVAL GLKGLNLYLS CVMKDGTPTL
QLESVDPKQY PKKKMEKRFV FNKIEVKTKV EFESAQFPNW
YISTSQAEHR PVFLGNSNGR DIVDFTMEPV SS

Measured Molecular Weight 17.3 kDa, observed by reducing SDS-PAGE.
Purity > 95% by SDS-PAGE and HPLC analyses.
Formulation Lyophilized after extensive dialysis against PBS.
Reconstitution Reconstituted in ddH2O at 100 μg/ml.
Endotoxin Level < 0.2 EU/μg, determined by LAL method.
Storage Lyophilized recombinant rat Interleukin-1 Beta (IL-1 Beta) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, recombinant rat IL-1 Beta should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.




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Cat. No. Z03014
Size
Price
50 ug $275.00
10 ug $85.00
1 mg $2100.00
 
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