|Synonyms||Fibroblast Growth Factor-basic, FGF-2, HBGF-2, Prostatropin; BFGF; FGFB;|
|Description||Fibroblast Growth Factor-basic (FGF-basic), also known as HBGF-2, is a non-glycosylated heparin-binding growth factor that belongs to the FGF family. FGF-basic is present in basement membranes and in the subendothelial extracellular matrix of blood vessels. FGF-basic signals through FGFR1, 2, 3 and 4 that plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration.
Recombinant mouse Fibroblast Growth Factor-basic (rmFGF-basic) produced in E. coli is a single non-glycosylated polypeptide chain containing 146 amino acids. A fully biologically active molecule, rmFGF-basic has a molecular mass of 16.4 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
|Biological Activity||ED50 < 0.5 ng/ml, measured by a cell proliferation assay using 3T3 Cells, corresponding to a specific activity of > 2.0× 106 units/mg.|
MPALPEDGGA AFPPGHFKDP KRLYCKNGGF FLRIHPDGRV
DGVREKSDPH VKLQLQAEER GVVSIKGVCA NRYLAMKEDG
RLLASKCVTE ECFFFERLES NNYNTYRSRK YSSWYVALKR
TGQYKLGSKT GPGQKAILFL PMSAKS
|Measured Molecular Weight||16.4 kDa, observed by reducing SDS-PAGE.|
|Purity||> 95% by SDS-PAGE and HPLC analyses.|
|Formulation||Lyophilized after extensive dialysis against PBS.|
|Reconstitution||Reconstituted in ddH2O at 100 μg/ml.|
|Endotoxin Level||< 0.2 EU/μg, determined by LAL method.|
|Storage||Lyophilized recombinant mouse Fibroblast Growth Factor-basic (rmFGF-basic) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, rmFGF-basic should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.|
|Note||For research use only|
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