|Synonyms||Cytokine synthesis inhibitory factor; CSIF; TGIF; IL10A|
|Description||Interleukin-10 (IL-10), also known as cytokine synthesis inhibitory factor (CSIF), is an anti-inflammatory cytokine produced by a variety of cell lines including T-cells, macrophages and mast cells. IL-10 is classified as a class-2 cytokine, a set of cytokines including IL-19, IL-20, IL-22, IL-24, and IL-26. IL-10 can inhibit the synthesis of pro-inflammatory cytokines such as IFN-gamma, IL-2, IL-3, TNF and GM-CSF. It also stimulates Th2 responses, but suppresses the antigen-presentation capacity of antigen presenting cells.
Recombinant rat Interleukin-10 (IL-10)produced in E. coli is a single non-glycosylated polypeptide chain containing 161 amino acids. A fully biologically active molecule, recombinant rat Interleukin-10 (IL-10) has a molecular mass of 18.7kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
|Biological Activity||ED50 < 0.5μg/ml, measured by a cell proliferation assay using C6 cells, corresponding to a specific activity of > 2.0× 103 units/mg.|
MSKGHSIKGD NNCTHFPVSQ THMLRELRAA FSQVKTFFQK
KDQLDNIVLT DSLLQDFKGY LGCQALSEMI KFYLVEVMPQ
AENHGPEIKE HLNSLGEKLK TLWIQLRRCH RFLPCENKSK
AVEQVKNDFN KLQDKGVYKA MNEFDIFINC IEAYVTLKMK
|Measured Molecular Weight||18.7 kDa, observed by reducing SDS-PAGE.|
|Purity||> 95% by SDS-PAGE and HPLC analyses.|
|Formulation||Lyophilized after extensive dialysis against PBS.|
|Reconstitution||Reconstituted in ddH2O at 100 μg/ml.|
|Endotoxin Level||< 0.2 EU/μg, determined by LAL method.|
|Storage||Lyophilized recombinant rat Interleukin-10 (IL-10) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, IL-10 should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.|
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