|Synonyms||Fibroblast Growth Factor-9, GAF (Glia-activating factor), HBGF-9|
|Description||Fibroblast Growth Factor-9 (FGF-9) is a heparin binding growth factor that belongs to the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF-9 was isolated as a secreted factor that exhibits a growth-stimulating effect on cultured glial cells. In nervous system, this protein is produced mainly by neurons and may be important for glial cell development.
Recombinant Human Fibroblast Growth Factor-9(rhFGF-9) produced in E. coli is a single non-glycosylated polypeptide chain containing 208 amino acids. A fully biologically active molecule, rhFGF-9 has a molecular mass of 23.4 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
|Biological Activity||ED50 < 2.0 ng/ml , measured by a cell proliferation assay using 3T3 cells, corresponding to a specific activity of >5.0× 105 units/mg.|
MAPLGEVGNY FGVQDAVPFG NVPVLPVDSP VLLSDHLGQS
EAGGLPRGPA VTDLDHLKGI LRRRQLYCRT GFHLEIFPNG
TIQGTRKDHS RFGILEFISI AVGLVSIRGV DSGLYLGMNE
KGELYGSEKL TQECVFREQF EENWYNTYSS NLYKHVDTGR
RYYVALNKDG TPREGTRTKR HQKFTHFLPR PVDPDKVPEL
|Measured Molecular Weight||23.4 kDa, observed by reducing SDS-PAGE.|
|Purity||> 95% by SDS-PAGE and HPLC analyses.|
|Formulation||Lyophilized after extensive dialysis against PBS.|
|Reconstitution||Reconstituted in ddH2O at 100 μg/ml.|
|Endotoxin Level||< 0.2 EU/μg, determined by LAL method.|
|Storage||Lyophilized recombinant Human Fibroblast Growth Factor-9(rhFGF-9) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, rhFGF-9 should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.|
|Note||For research use only|
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