Synonyms | Fibroblast Growth Factor-9, GAF (Glia-activating factor), HBGF-9 |
Description | Fibroblast Growth Factor-9 (FGF-9) is a heparin binding growth factor that belongs to the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF-9 was isolated as a secreted factor that exhibits a growth-stimulating effect on cultured glial cells. In nervous system, this protein is produced mainly by neurons and may be important for glial cell development. Recombinant Human Fibroblast Growth Factor-9(rhFGF-9) produced in E. coli is a single non-glycosylated polypeptide chain containing 208 amino acids. A fully biologically active molecule, rhFGF-9 has a molecular mass of 23.4 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript. |
Accession No | P31371 |
Species | Human |
Source | E. coli |
Biological Activity | ED50 < 2.0 ng/ml , measured by a cell proliferation assay using 3T3 cells, corresponding to a specific activity of >5.0× 105 units/mg. |
Sequence | MAPLGEVGNY FGVQDAVPFG NVPVLPVDSP VLLSDHLGQS EAGGLPRGPA VTDLDHLKGI LRRRQLYCRT GFHLEIFPNG TIQGTRKDHS RFGILEFISI AVGLVSIRGV DSGLYLGMNE KGELYGSEKL TQECVFREQF EENWYNTYSS NLYKHVDTGR RYYVALNKDG TPREGTRTKR HQKFTHFLPR PVDPDKVPEL YKDILSQS |
Measured Molecular Weight | 23.4 kDa, observed by reducing SDS-PAGE. |
Purity | > 95% by SDS-PAGE and HPLC analyses. |
Formulation | Lyophilized after extensive dialysis against PBS. |
Reconstitution | Reconstituted in ddH2O at 100 μg/ml. |
Endotoxin Level | < 0.2 EU/μg, determined by LAL method. |
Storage | Lyophilized recombinant Human Fibroblast Growth Factor-9(rhFGF-9) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, rhFGF-9 should be stable up to 2 weeks at 4°C or up to 3 months at -20°C. |
Note | For research use only |
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