|Synonyms||Fibroblast Growth Factor-10, FGFA, Keratinocyte growth factor-2|
|Description||Fibroblast Growth Factor-10 (FGF-10) is a mitogen mainly produced by mesenchymal stem cells in lung. FGF-10 belongs to the heparin binding FGF family, and is also known as Keratinocyte Growth Factor-2 (KGF-2). It shares the homolog and receptor FGFR2-IIIb with KGF. However, unlike KGF which induces the proliferation and differentiation of various epithelial cells, FGF-10 is an essential factor for the budding and branching morphogenesis during the multi-organ development via the instructive mesenchymal-epithelial interactions. FGF-10 is crucial for lung and limb development, and is regulated by Shh during early development.
Recombinant human Fibroblast Growth Factor-10 (rhFGF-10) with N-terminal His-tag produced in E. coli is a single non-glycosylated polypeptide chain containing 187 amino acids. A fully biologically active molecule, rhFGF-10 has a molecular mass of 21.4 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
|Biological Activity||ED50 < 20 ng/mL, measured by a cell proliferation assay using 4MBr-5 cells, corresponding to a specific activity of > 5.0× 104 units/mg.|
MNHKVHHHHH HMDDDDKMLG QDMVSPEATN SSSSSFSSPS
SAGRHVRSYN HLQGDVRWRK LFSFTKYFLK IEKNGKVSGT
KKENCPYSIL EITSVEIGVV AVKAINSNYY LAMNKKGKLY
GSKEFNNDCK LKERIEENGY NTYASFNWQH NGRQMYVALN
GKGAPRRGQK TRRKNTSAHF LPMVVHS
|Measured Molecular Weight||21.4 kDa, observed by reducing SDS-PAGE.|
|Purity||> 95% by SDS-PAGE analysis.|
|Formulation||Lyophilized after extensive dialysis against PBS.|
|Reconstitution||Reconstituted in ddH2O at 100 μg/mL.|
|Endotoxin Level||< 0.2 EU/μg, determined by LAL method.|
|Storage||Lyophilized recombinant human Fibroblast Growth Factor-10 (rhFGF-10) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, rhFGF-10 should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.|
|Note||For research use only|
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