|Synonyms||Stem Cell Factor, c-Kit Ligand, Mast Cell Growth Factor (MGF), Steel Factor|
|Description||Stem Cell Factor (SCF) is a hematopoietic growth factor that binds to the c-Kit receptor. SCF exerts its activity during the early stages of hematopoiesis. SCF stimulates the proliferation of myeloid, erythroid, and lymphoid progenitors in bone marrow cultures and has been shown to act synergistically with colony stimulating factors.
Recombinant rat Stem Cell Factor (rrSCF) produced in P. pastoris is a single glycosylated polypeptide chain containing 164 amino acids. A fully biologically active molecule, rrSCF has a molecular mass of 18.3 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
|Biological Activity||ED50 < 25 ng/mL, measured by a cell proliferation assay using TF-1 Cells, corresponding to a specific activity of > 4× 104 units/mg.|
QEICRNPVTD NVKDITKLVA NLPNDYMITL NYVAGMDVLP
SHCWLRDMVT HLSVSLTTLL DKFSNISEGL SNYSIIDKLG
KIVDDLVACM EENAPKNVKE SLKKPETRNF TPEEFFSIFN
RSIDAFKDFM VASDTSDCVL SSTLGPEKDS RVSVTKPFML
|Measured Molecular Weight||18.3 kDa, observed by reducing SDS-PAGE.|
|Purity||> 95% by SDS-PAGE analysis.|
|Formulation||Lyophilized after extensive dialysis against PBS.|
|Reconstitution||Reconstituted in ddH2O at 100 μg/mL.|
|Endotoxin Level||< 0.2 EU/μg, determined by LAL method.|
|Storage||Lyophilized recombinant rat Stem Cell Factor (rrSCF) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, rrSCF should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.|
|Note||For research use only|
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